Akcali Kamil Can, Dalgic Aydin, Ucar Ahmet, Haj Khemaeis Ben, Guvenc Dilek
Department of Molecular Biology and Genetics, Bilkent University, Bilkent, Ankara 06800, Turkey.
World J Gastroenterol. 2004 Jan 15;10(2):279-83. doi: 10.3748/wjg.v10.i2.279.
During liver regeneration cellular proliferation and apoptosis result in tissue remodeling to restore normal hepatic mass and structure. Main regulators of the apoptotic machinery are the Bcl-2 family proteins but their roles are not well defined throughout the liver regeneration. We aimed to analyze the expression levels of bcl-2 gene family members during resection induced liver regeneration.
We performed semi-quantitative RT-PCR to examine the expression level of bak, bax, bcl-2 and bcl-xL in 70% hepatectomized rat livers during the whole regeneration process and compared to that of the sham and normal groups.
The expression of bak and bax was decreased whereas that of bcl-2 and bcl-xL was increased in hepatectomized animals compared to normal liver at most time points. We also reported for the first time that sham group of animals had statistically significant higher expression of bak and bax than hepatectomized animals. In addition, the area under the curve (AUC) values of these genes was larger in sham groups than the hepatectomized groups.
The expression changes of bak, bax, bcl-2 and bcl-xL genes are altered not only due to regeneration, but also due to effects of surgical operations.
在肝脏再生过程中，细胞增殖和凋亡导致组织重塑，以恢复正常的肝脏质量和结构。凋亡机制的主要调节因子是Bcl-2家族蛋白，但其在整个肝脏再生过程中的作用尚未明确。我们旨在分析肝切除诱导的肝脏再生过程中bcl-2基因家族成员的表达水平。
我们进行了半定量RT-PCR，以检测70%肝切除大鼠肝脏在整个再生过程中bak、bax、bcl-2和bcl-xL的表达水平，并与假手术组和正常组进行比较。
与正常肝脏相比，在大多数时间点，肝切除动物中bak和bax的表达降低，而bcl-2和bcl-xL的表达增加。我们还首次报道，假手术组动物中bak和bax的表达在统计学上显著高于肝切除动物。此外，这些基因在假手术组中的曲线下面积（AUC）值大于肝切除组。
bak、bax、bcl-2和bcl-xL基因的表达变化不仅是由于再生，还受到手术操作的影响。
