秀丽隐杆线虫的微小RNA let-7与lin-41 3'非翻译区中不完全的let-7互补位点结合。
The C. elegans microRNA let-7 binds to imperfect let-7 complementary sites from the lin-41 3'UTR.
作者信息
Vella Monica C, Choi Eun-Young, Lin Shin-Yi, Reinert Kristy, Slack Frank J
机构信息
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
出版信息
Genes Dev. 2004 Jan 15;18(2):132-7. doi: 10.1101/gad.1165404. Epub 2004 Jan 16.
Abstract
Caenorhabditis elegans let-7, a founding member of the microRNA family, is predicted to bind to six sites in the 3'UTR of the mRNA of its target gene, lin-41, to down-regulate LIN-41. Here, we demonstrate that wild-type let-7 microRNA binds in vitro to RNA from the lin-41 3'UTR. This interaction is dependent on two conserved let-7 complementary sites (LCSs). A 27-nucleotide sequence between the LCSs is also necessary for down-regulation in vivo. LCS mutations compensatory to the lesion in let-7(n2853) can partially restore lin-41 3'UTR function in a let-7(n2853) background, providing the first experimental evidence for an animal miRNA binding directly to its validated target in vivo.
摘要
秀丽隐杆线虫的let-7是微小RNA家族的创始成员，预计可与靶基因lin-41的mRNA的3'非翻译区中的六个位点结合，从而下调LIN-41。在此，我们证明野生型let-7微小RNA在体外与lin-41 3'非翻译区的RNA结合。这种相互作用依赖于两个保守的let-7互补位点（LCS）。LCS之间的一个27个核苷酸的序列对于体内下调也是必需的。与let-7(n2853)中的损伤互补的LCS突变可以在let-7(n2853)背景中部分恢复lin-41 3'非翻译区的功能，为动物微小RNA在体内直接与其经过验证的靶标结合提供了首个实验证据。
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本文引用的文献
1
The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics.秀丽隐杆线虫的基因组序列：一个用于比较基因组学的平台。
PLoS Biol. 2003 Nov;1(2):E45. doi: 10.1371/journal.pbio.0000045. Epub 2003 Nov 17.
2
Embryonic stem cell-specific MicroRNAs.胚胎干细胞特异性微小RNA
Dev Cell. 2003 Aug;5(2):351-8. doi: 10.1016/s1534-5807(03)00227-2.
3
The time of appearance of the C. elegans let-7 microRNA is transcriptionally controlled utilizing a temporal regulatory element in its promoter.秀丽隐杆线虫let-7微小RNA的出现时间是通过其启动子中的一个时序调控元件进行转录控制的。
Dev Biol. 2003 Jul 15;259(2):364-79. doi: 10.1016/s0012-1606(03)00202-1.
4
The C elegans hunchback homolog, hbl-1, controls temporal patterning and is a probable microRNA target.秀丽隐杆线虫驼背同源物hbl-1控制时间模式，并且可能是一种微小RNA的靶标。
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5
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Curr Biol. 2003 Apr 29;13(9):790-5. doi: 10.1016/s0960-9822(03)00250-1.
6
bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila.“短脚鸡”编码一种在发育过程中受到调控的微小RNA，它控制细胞增殖并调节果蝇中促凋亡基因“头部 involution defective”（hid）。
Cell. 2003 Apr 4;113(1):25-36. doi: 10.1016/s0092-8674(03)00231-9.
7
The microRNAs of Caenorhabditis elegans.秀丽隐杆线虫的微小RNA
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