用海洋细菌酶消化冈村枝管藻岩藻依聚糖衍生的低聚糖的结构
Structures of oligosaccharides derived from Cladosiphon okamuranus fucoidan by digestion with marine bacterial enzymes.
作者信息
Sakai Takeshi, Ishizuka Kumiko, Shimanaka Kazuo, Ikai Katsushige, Kato Ikunoshin
机构信息
Takara Bio Inc, Biotechnology Research Laboratories, Seta 3-4-1, Otsu-shi, Shiga-ken 520-2193, Japan.
出版信息
Mar Biotechnol (NY). 2003 Nov-Dec;5(6):536-44. doi: 10.1007/s10126-002-0107-9. Epub 2003 Jul 17.
A fucoidan-utilizing marine bacterium, Fucophilus fucoidanolyticus, was cultivated in medium containing fucoidan from Cladosiphon okamuranus. The C. okamuranus fucoidan was digested into oligosaccharides with the intracellular enzymes of F. fucoidanolyticus, and their structures were determined by nuclear magnetic resonance analyses. Some of their structures are represented by one general structural formula, (-3 L-Fuc palpha1-3 L-Fuc p(4- O-sulfate)alpha1-3 L-Fuc p(4- O-sulfate)alpha1-3( D-Glc pUAalpha1-2) L-Fuc palpha1)(m)-3 L-Fuc palpha1-3 L-Fuc p(4- O-sulfate)alpha1-3 L-Fuc p(4- O-sulfate) alpha1-3 L-Fuc p ( m = 0, 1, 2, or 3). We concluded that all oligosaccharides obtained were derived from a sulfated-fucose-containing polysaccharide of C. okamuranus, which has a repeating unit of (-3 L-Fuc palpha1-3 L-Fuc p(4- O-sulfate)alpha1-3 L-Fuc p(4- O-sulfate)alpha1-3( D-Glc pUAalpha1-2) L-Fuc palpha1-).
一种利用岩藻依聚糖的海洋细菌——岩藻依聚糖分解嗜岩藻菌（Fucophilus fucoidanolyticus），在含有来自冈村枝管藻（Cladosiphon okamuranus）的岩藻依聚糖的培养基中培养。冈村枝管藻岩藻依聚糖被岩藻依聚糖分解嗜岩藻菌的胞内酶消化成寡糖，其结构通过核磁共振分析确定。它们的一些结构由一个通用结构式表示：(-3 L-岩藻糖α1-3 L-岩藻糖基（4-O-硫酸酯）α1-3 L-岩藻糖基（4-O-硫酸酯）α1-3（D-葡萄糖醛酸α1-2）L-岩藻糖α1)(m)-3 L-岩藻糖α1-3 L-岩藻糖基（4-O-硫酸酯）α1-3 L-岩藻糖基（4-O-硫酸酯）α1-3 L-岩藻糖基（m = 0、1、2或3）。我们得出结论，所有获得的寡糖均源自冈村枝管藻的一种含硫酸化岩藻糖的多糖，其重复单元为(-3 L-岩藻糖α1-3 L-岩藻糖基（4-O-硫酸酯）α1-3 L-岩藻糖基（4-O-硫酸酯）α1-3（D-葡萄糖醛酸α1-2）L-岩藻糖α1-) 。
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