一氧化氮（NO）信号通路对KCC3a和KCC3b信使核糖核酸（mRNA）的表达有不同的调节作用。
The NO signaling pathway differentially regulates KCC3a and KCC3b mRNA expression.
作者信息
Di Fulvio Mauricio, Lauf Peter K, Adragna Norma C
机构信息
Department of Pharmacology and Toxicology, Wright State University, School of Medicine, 3640 Colonel Glenn Highway, Biological Sciences Building, Room 152-6, Dayton, OH 45435-0002, USA.
出版信息
Nitric Oxide. 2003 Nov;9(3):165-71. doi: 10.1016/j.niox.2003.11.004.
Nitric oxide (NO) donors and protein kinase G (PKG) acutely up-regulate K-Cl cotransporter-1 and -3 (KCC1 and KCC3) mRNA expression in vascular smooth muscle cells (VSMCs). Here, we report the presence, relative abundance, and regulation by sodium nitroprusside (SNP) of the novel KCC3a and KCC3b mRNAs, in primary cultures of rat VSMCs. KCC3a and KCC3b mRNAs were expressed in an approximate 3:1 ratio, as determined by semiquantitative RT-PCR analysis. SNP as well as YC-1 and 8-Br-cGMP, a NO-independent stimulator of soluble guanylyl cyclase (sGC) and PKG, respectively, increased KCC3a and KCC3b mRNA expression by 2.5-fold and 8.1-fold in a time-dependent manner, following a differential kinetics. Stimulation of the NO/sGC/PKG signaling pathway with either SNP, YC-1, or 8-Br-cGMP decreased the KCC3a/KCC3b ratio from 3.0+/-0.4 to 0.9+/-0.1. This is the first report on a differential regulation by the NO/sGC/PKG signaling pathway of a cotransporter and of KCC3a and KCC3b mRNA expression.
一氧化氮（NO）供体和蛋白激酶G（PKG）可急性上调血管平滑肌细胞（VSMC）中钾氯共转运体-1和-3（KCC1和KCC3）的mRNA表达。在此，我们报告了大鼠VSMC原代培养物中新型KCC3a和KCC3b mRNA的存在、相对丰度以及硝普钠（SNP）对其的调节作用。通过半定量RT-PCR分析确定，KCC3a和KCC3b mRNA以大约3:1的比例表达。SNP以及分别作为可溶性鸟苷酸环化酶（sGC）和PKG的非NO依赖性刺激剂的YC-1和8-溴-cGMP，以时间依赖性方式分别使KCC3a和KCC3b mRNA表达增加2.5倍和8.1倍，呈现出不同的动力学。用SNP、YC-1或8-溴-cGMP刺激NO/sGC/PKG信号通路，可使KCC3a/KCC3b比值从3.0±0.4降至0.9±0.1。这是关于NO/sGC/PKG信号通路对共转运体以及KCC3a和KCC3b mRNA表达的差异调节的首次报道。
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