Ahuja S K, Ferreira G M, Moreira A R
Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA.
J Ind Microbiol Biotechnol. 2004 Jan;31(1):41-7. doi: 10.1007/s10295-004-0113-1. Epub 2004 Jan 24.
The nutritional behavior of a cellulolytic nitrogen-fixing shipworm bacterium, Teredinobacter turnirae, is described, with respect to various carbon and nitrogen sources, in terms of endoglucanase production. Also, the effects of various surfactants on enzyme production are reported. Among the carbon sources, sucrose results in the maximum enzyme production, followed by cellulose. Ammonium phosphate proves to be the best nitrogen source for endoglucanase production. Various surfactants enhance the enzyme titers, with Triton X-100 yielding the best results. A combination of the above-mentioned components improves the enzyme production by 3.6-fold.
本文描述了一种纤维素分解固氮船蛆细菌——特纳氏船蛆杆菌(Teredinobacter turnirae)在各种碳源和氮源方面的营养行为,以内切葡聚糖酶的产生来衡量。此外,还报道了各种表面活性剂对酶产生的影响。在碳源中,蔗糖导致酶产量最高,其次是纤维素。磷酸铵被证明是内切葡聚糖酶产生的最佳氮源。各种表面活性剂提高了酶活性,其中吐温X-100效果最佳。上述成分的组合使酶产量提高了3.6倍。