溴化乙锭与DNA的结合：与单链和双链结构的相互作用。
The binding of ethidium bromide with DNA: interaction with single- and double-stranded structures.
作者信息
Vardevanyan P O, Antonyan A P, Parsadanyan M A, Davtyan H G, Karapetyan A T
机构信息
Biophysics Department of the Biological, Faculty of Yerevan State University, 375025 Yerevan, Aleck Manoogian St. 1, Armenia.
出版信息
Exp Mol Med. 2003 Dec 31;35(6):527-33. doi: 10.1038/emm.2003.68.
The pH-induced helix-coil transition of DNA and its complexes with EtBr is carried out at acidic pH in a wide interval of change of concentration ratio of EtBr/DNA. The binding isotherms of EtBr on double and single-stranded DNA at pH = 7.0 and pH = 3.0 (t = 25(o)C) are obtained by absorption and fluorimetric methods. Binding constants (K) and number of bases (n), corresponding to one binding site were determined. Non fluorescent "strong" complex with ds-DNA at pH = 7.0 and t = 25(o)C as well as "strong" and "weak" complexes with ss-DNA at pH = 3.0 and t = 25(o)C are revealed.
DNA及其与溴化乙锭（EtBr）复合物的pH诱导螺旋-线圈转变是在酸性pH条件下，于EtBr/DNA浓度比变化的较宽范围内进行的。通过吸收和荧光法获得了pH = 7.0和pH = 3.0（t = 25°C）时EtBr在双链和单链DNA上的结合等温线。测定了对应于一个结合位点的结合常数（K）和碱基数（n）。揭示了在pH = 7.0和t = 25°C时与双链DNA形成的非荧光“强”复合物，以及在pH = 3.0和t = 25°C时与单链DNA形成的“强”和“弱”复合物。
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