DBA/2J小鼠（一种高眼压模型）中视网膜犬尿烯酸和犬尿氨酸转氨酶随年龄的降低

Age-dependent decrease of retinal kynurenate and kynurenine aminotransferases in DBA/2J mice, a model of ocular hypertension.

作者信息

Rejdak Robert, Kohler Konrad, Kocki Tomasz, Shenk Yana, Turski Waldemar A, Okuno Etsuo, Lehaci Cristiana, Zagorski Zbigniew, Zrenner Eberhart, Schuettauf Frank

机构信息

Department of Pathophysiology of Vision and Neuro-Ophthalmology, Division of Experimental Opthalmology, University Eye Hospital, Röntgenweg 11, 72076 Tübingen, Germany.

出版信息

Vision Res. 2004 Mar;44(7):655-60. doi: 10.1016/j.visres.2003.11.003.

DOI:10.1016/j.visres.2003.11.003

PMID:14751550

Abstract

The study examines age-dependent changes of kynurenic acid (KYNA) content and kynurenine aminotransferases (KAT I and KAT II) celluar expression in the retinas of DBA/2J mice. Retinas were obtained from DBA/2J mice of different ages (3, 6 and 11 months). C57BL6 mice were used as controls. As measured with HPLC, KYNA content decreased (p < 0.01) in the retinas of 6-month-old DBA/2J mice and continued to decrease (p < 0.0074) in the retinas of 11-month-old animals compared to the controls. Immunohistochemistry showed that expression of both KAT I and KAT II decreased markedly in the retinas of 11-month-old DBA/2J mice compared to controls. The impairment in KYNA biosynthesis in the retinas of DBA/2J mice may be one of the mechanisms of retinal neurodegeneration related to ocular hypertension.

摘要

该研究检测了DBA/2J小鼠视网膜中犬尿喹啉酸（KYNA）含量以及犬尿氨酸转氨酶（KAT I和KAT II）的细胞表达随年龄的变化情况。从不同年龄（3、6和11个月）的DBA/2J小鼠获取视网膜。以C57BL6小鼠作为对照。通过高效液相色谱法测定，与对照组相比，6月龄DBA/2J小鼠视网膜中的KYNA含量降低（p < 0.01），11月龄动物视网膜中的KYNA含量继续降低（p < 0.0074）。免疫组织化学显示，与对照组相比，11月龄DBA/2J小鼠视网膜中KAT I和KAT II的表达均显著降低。DBA/2J小鼠视网膜中KYNA生物合成受损可能是与高眼压相关的视网膜神经变性的机制之一。