与β-淀粉样蛋白相关的蛋白早老素1和β-分泌酶1被轴突运输至大脑中的神经末梢。
The beta-amyloid-related proteins presenilin 1 and BACE1 are axonally transported to nerve terminals in the brain.
作者信息
Sheng Jin G, Price Donald L, Koliatsos Vassilis E
机构信息
Division of Neuropathology and the Alzheimer's Disease Research Center, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
出版信息
Exp Neurol. 2003 Dec;184(2):1053-7. doi: 10.1016/j.expneurol.2003.08.018.
In this study, we show that removal of entorhinal cortex (ERC) afferents to hippocampus reduces levels of presenilin 1 (PS1) in the dentate gyrus of APPswe/PS1DeltaE9 transgenic (Tg) mice. PS1 immunoreactivity on the deafferented dentate gyrus decreases by approximately 25% and 50%, 2 and 4 weeks post-lesion compared to the contralateral side; by Western blotting, there is an approximately 40% decrease of the 43 kDa (full length) PS1 and an approximately 80% decrease of the 28 kDa (N-terminal fragment) PS1 on the lesioned dentate gyrus. Levels of beta-site APP Cleavage Enzyme 1 (BACE1) immunoreactivity also decrease by approximately 50% and 65% 2 and 4 weeks post-lesion. Together, these data demonstrate that PS1 and BACE1 are transported from the entorhinal cortex to the hippocampus via axons of the perforant pathway.
在本研究中，我们发现去除内嗅皮质（ERC）至海马的传入纤维可降低APPswe/PS1DeltaE9转基因（Tg）小鼠齿状回中早老素1（PS1）的水平。与对侧相比，去传入纤维的齿状回上PS1免疫反应性在损伤后2周和4周分别降低约25%和50%；通过蛋白质印迹法，损伤的齿状回上43 kDa（全长）PS1约减少40%，28 kDa（N端片段）PS1约减少80%。β位点APP裂解酶1（BACE1）免疫反应性水平在损伤后2周和4周也分别降低约50%和65%。这些数据共同表明，PS1和BACE1通过穿通通路的轴突从内嗅皮质运输至海马。
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