蜂毒肽与二肉豆蔻酰磷脂酰甘油双层膜相互作用的高压傅里叶变换红外光谱研究

High pressure FTIR study of interaction of melittin with dimyristoylphosphatidyl glycerol bilayers.

作者信息

Ahmed M K, Choma C T, Wong P T

机构信息

Steacie Institute for Molecular Sciences, National Research Council of Canada, Ottawa, Ontario.

出版信息

Chem Phys Lipids. 1992 Nov;63(1-2):139-48. doi: 10.1016/0009-3084(92)90030-s.

DOI:10.1016/0009-3084(92)90030-s

PMID:1486656

Abstract

Infrared spectra of hydrated dimyristoylphosphatidyl glycerol (DMPG) and of aqueous dispersions of melittin and DMPG at peptide:lipid molar ratios of 1:10 and 1:4 were recorded as a function of pressure from atmospheric to 22 kbar. Spectral features corresponding to vibrations of the amide linkages in melittin and to various functional groups in DMPG (carbonyl, methlylene, phosphate) were monitored in order to investigate the structure and dynamics of melittin:DMPG dispersions. Melittin was found to cause conformational and orientational disordering of the acyl chains in DMPG bilayers. The magnitude of these disorders was higher for higher concentration of melittin in DMPG. The higher concentration of melittin was also found to disrupt the DMPG bilayers through interactions with the lipid head groups. Such disruption may be related to some of the biological properties of melittin.

摘要

记录了水合二肉豆蔻酰磷脂酰甘油（DMPG）以及蜂毒肽与DMPG在肽:脂质摩尔比为1:10和1:4时的水分散体在从常压到22千巴的压力范围内的红外光谱。监测了与蜂毒肽中酰胺键振动以及DMPG中各种官能团（羰基、亚甲基、磷酸基团）相对应的光谱特征，以研究蜂毒肽:DMPG分散体的结构和动力学。发现蜂毒肽会导致DMPG双层中酰基链的构象和取向无序。在DMPG中蜂毒肽浓度越高，这些无序的程度越高。还发现蜂毒肽的较高浓度会通过与脂质头部基团的相互作用破坏DMPG双层。这种破坏可能与蜂毒肽的一些生物学特性有关。