对小型神经元区室中的钙浓度动态进行成像。
Imaging calcium concentration dynamics in small neuronal compartments.
作者信息
Yasuda Ryohei, Nimchinsky Esther A, Scheuss Volker, Pologruto Thomas A, Oertner Thomas G, Sabatini Bernardo L, Svoboda Karel
机构信息
Howard Hughes Medical Institute, The Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
出版信息
Sci STKE. 2004 Feb 3;2004(219):pl5. doi: 10.1126/stke.2192004pl5.
Calcium and its regulation play central roles diverse physiologic processes. Quantification of calcium concentrations ([Ca2+]) in small neuronal compartments is crucial to understanding Ca2+-dependent signaling. Here, we describe techniques that are optimized for 2-photon imaging of [Ca2+] dynamics in small compartments such as dendrites and dendritic spines.
钙及其调节在多种生理过程中发挥着核心作用。对小神经元区室中钙浓度（[Ca2+]）进行定量，对于理解钙依赖性信号传导至关重要。在此，我们描述了针对树突和树突棘等小区室中[Ca2+]动态的双光子成像进行优化的技术。
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