Tashtoush B M, Alali F Q, Najib N M
Department of Pharmaceutical Technology, College of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.
Pharmazie. 2004 Jan;59(1):21-4.
A validated liquid chromatographic-mass spectrometric (LC/MS) method for the determination of lisinopril in human plasma is presented. Enalapril was used as an internal standard. After the addition of internal standard, solid phase extraction was used as a cleaning step. To separate lisinopril and enalapril from interfering endogenous plasma substances, the analysis was performed using column switching valve. The quantitative determination was performed using selected ion monitoring (+)-electrospray LC-MS. A combination of an acidic mobile phase and a reverse phase column was used. A precision in the linear range from 10 to 500.0 ng/mL plasma, absolute recovery of 91.69% for lisinopril and 90.26% for enalapril, stability for 3.5 months at -20 degrees C have been achieved. Limit of quantitation (LOQ) was 10 ng/mL while limit of detection (LOD) was about 1 ng/mL.
本文介绍了一种经过验证的用于测定人血浆中赖诺普利的液相色谱-质谱（LC/MS）方法。依那普利用作内标。加入内标后，采用固相萃取作为净化步骤。为了将赖诺普利和依那普利与干扰性内源性血浆物质分离，使用柱切换阀进行分析。采用选择离子监测（+）-电喷雾LC-MS进行定量测定。使用了酸性流动相和反相柱的组合。在10至500.0 ng/mL血浆的线性范围内具有精密度，赖诺普利的绝对回收率为91.69%，依那普利的绝对回收率为90.26%，在-20℃下可稳定保存3.5个月。定量限（LOQ）为10 ng/mL，检测限（LOD）约为1 ng/mL。
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