泰国儿童腹泻相关大肠杆菌的患病率。

Prevalence of childhood diarrhoea-associated Escherichia coli in Thailand.

作者信息

Ratchtrachenchai Orn-Anong, Subpasu Sarayoot, Hayashi Hideo, Ba-Thein William

机构信息

Enteric Laboratory, National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Tiwanonth Road, Amphur Muang Nonthaburi, 11000, Thailand 2Department of Human Nutrition, Faculty of Contemporary Sciences, Chugoku-Gakuen University, 83 Niwase, Okayama, 701-0197, Japan 3Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, 305-8575, Japan.

出版信息

J Med Microbiol. 2004 Mar;53(Pt 3):237-243. doi: 10.1099/jmm.0.05413-0.

DOI:10.1099/jmm.0.05413-0

PMID:14970250

Abstract

Escherichia coli isolates (n=2629) were collected between 1996 and 2000 from 2100 Thai children less than 12 years of age with acute diarrhoea. Enterotoxigenic (ETEC), enteroinvasive (EIEC), Shiga-toxin-producing (STEC), enteropathogenic (EPEC) and enteroaggregative (EAEC) E. coli were identified by their virulence marker profiles, as determined by multiplex PCR, and HeLa cell-adherence patterns. Serogroups of isolates were determined using 43 monovalent O antisera. Of 2629 isolates, 16.9% were identified as diarrhoeagenic E. coli, and the mean isolation rates per year were 10.2% for EAEC (range 8-12.5%), 3.2% for EPEC (0-8%), 3.0% for ETEC (2-5.4%), 0.5% for EIEC (0-1%) and 0.04 % for STEC (0-0.1%). The isolation rates of pathotypes from four different age groups (0-5 months, 6-11 months, 1-2 years and 2-12 years) in 905 children whose ages were recorded were respectively 19.3, 18.2, 9.1 and 8.1% for EAEC, 3.1, 4.3, 1.7 and 2.2% for EPEC and 2.6, 2.3, 1.3 and 5% for ETEC. About 38% of diarrhoeagenic E. coli, including 55.1, 66.7, 100, 45.9 and 29%, respectively, of ETEC, EIEC, STEC, EPEC and EAEC, and 24% of non-diarrhoeagenic E. coli were O-antigen typable. Only four serogroups (9.3%) were restricted to single pathotypes, whereas 27 serogroups (62.8%) were not restricted to any pathotype. This study shows that EAEC are the most prevalent diarrhoea-associated pathotype in Thai children.

摘要

1996年至2000年间，从2100名12岁以下患急性腹泻的泰国儿童中收集了2629株大肠杆菌分离株。通过多重聚合酶链反应（PCR）测定的毒力标记谱和HeLa细胞黏附模式，鉴定产肠毒素性大肠杆菌（ETEC）、侵袭性大肠杆菌（EIEC）、产志贺毒素大肠杆菌（STEC）、致病性大肠杆菌（EPEC）和聚集性大肠杆菌（EAEC）。使用43种单价O抗血清确定分离株的血清群。在2629株分离株中，16.9%被鉴定为致泻性大肠杆菌，每年的平均分离率分别为：EAEC为10.2%（范围8 - 12.5%），EPEC为3.2%（0 - 8%），ETEC为3.0%（2 - 5.4%），EIEC为0.5%（0 - 1%），STEC为0.04%（0 - 0.1%）。在记录了年龄的905名儿童中，四个不同年龄组（0 - 5个月、6 - 11个月、1 - 2岁和2 - 12岁）致病型的分离率分别为：EAEC为19.3%、18.2%、9.1%和8.1%，EPEC为3.1%、4.3%、1.7%和2.2%，ETEC为2.6%、2.3%、1.3%和5%。约38%的致泻性大肠杆菌，包括ETEC、EIEC、STEC、EPEC和EAEC分别为55.1%、66.7%、100%、45.9%和29%，以及24%的非致泻性大肠杆菌可进行O抗原分型。只有四个血清群（9.3%）局限于单一致病型，而27个血清群（62.8%）不局限于任何致病型。这项研究表明，EAEC是泰国儿童中最常见的与腹泻相关的致病型。