通过乌吉四组分反应合成簇状甘露糖苷及其对酵母甘露聚糖与伴刀豆球蛋白A结合的抑制作用。

Synthesis of cluster mannosides via a Ugi four-component reaction and their inhibition against the binding of yeast mannan to concanavalin A.

作者信息

Li Yingxia, Zhang Xiaoru, Chu Shidong, Yu Kunyu, Guan Huashi

机构信息

Marine Drug and Food Institute, Ocean University of China, Qingdao 266003, China.

出版信息

Carbohydr Res. 2004 Mar 15;339(4):873-9. doi: 10.1016/j.carres.2003.12.005.

DOI:10.1016/j.carres.2003.12.005

PMID:14980831

Abstract

The Ugi four-component reaction (U-4CR) was utilized to prepare divalent and trivalent cluster mannosides with different scaffolds. The glycoclusters obtained were tested for their relative inhibitory potency against the binding of yeast mannan to concanavalin A by solid-phase enzyme-linked lectin assays (ELLA) using methyl alpha-D-mannopyranoside as a standard. Among them, a divalent mannoside containing aromatic groups showed the strongest binding affinity to concanavalin A.

摘要

利用乌吉四组分反应（U-4CR）制备了具有不同支架的二价和三价簇甘露糖苷。通过使用α-D-吡喃甘露糖苷作为标准品的固相酶联凝集素测定法（ELLA），对所获得的糖簇进行了针对酵母甘露聚糖与伴刀豆球蛋白A结合的相对抑制效力测试。其中，一种含有芳基的二价甘露糖苷对伴刀豆球蛋白A表现出最强的结合亲和力。