[胶原结合试验在血管性血友病中的应用]

[Application of collagen-binding assay for von Willebrand disease].

作者信息

Zhou Jing, Jia Yong-qian, Jiang Hong, Yang Yi-ming, Deng Cheng-qi

机构信息

Department of Clinical Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2004 Jan;35(1):126-9.

PMID:14981837

Abstract

OBJECTIVE

To explore the application of collagen-binding assay for von Willebrand disease (vWD).

METHODS

We investigated the sensitivity and reproducibility of collagen-binding assays (CBA) of vWF using the enzyme-linked immunosorbent assay (ELISA) technique and compared CBA with 3 other assays for their ability to detect vWD.

RESULTS

It was found that the vWF: CBA level of plasma from patients with different types of vWD is lower than that from healthy donors, hemophilia and other bleeding disorders (P < 0.001). In general, the four assays have the capability to identify vWD from normal and other bleeding disorders, and can be used for diagnosing vWD. The concordance rates of the four assays on vWD are vWF: Ag 85.7%, vWF: Rcof 76.2%, RIPA 80.9%, vWF: CBA 95.2% respectively.

CONCLUSION

vWF: CBA measured by ELISA is highly sensitive, specific and reproducible, and easy to operate. The measurement of the functional activity of vWF by vWF: Rcof or RIPA can be replaced by the more reliable vWF: CBA.

摘要

目的

探讨胶原结合试验在血管性血友病（vWD）中的应用。

方法

我们采用酶联免疫吸附测定（ELISA）技术研究血管性血友病因子（vWF）胶原结合试验（CBA）的敏感性和可重复性，并将CBA与其他3种检测方法检测vWD的能力进行比较。

结果

发现不同类型vWD患者血浆的vWF:CBA水平低于健康供者、血友病患者及其他出血性疾病患者（P<0.001）。总体而言，这四种检测方法均有能力从正常人和其他出血性疾病中鉴别出vWD，可用于vWD的诊断。这四种检测方法对vWD的符合率分别为：vWF:Ag 85.7%、vWF:Rcof 76.2%、RIPA 80.9%、vWF:CBA 95.2%。