燕麦坏死斑驳病毒的全核苷酸序列：一种与小麦条纹花叶病毒关系最为密切的独特小麦病毒属病毒（马铃薯Y病毒科）
Complete nucleotide sequence of Oat necrotic mottle virus: a distinct Tritimovirus species (family Potyviridae) most closely related to Wheat streak mosaic virus.
作者信息
Stenger D C, French R
机构信息
United States Department of Agriculture, Agricultural Research Service and Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska, USA.
出版信息
Arch Virol. 2004 Mar;149(3):633-40. doi: 10.1007/s00705-003-0237-z. Epub 2003 Nov 26.
The RNA genome (9346 nucleotides) of Oat necrotic mottle virus (ONMV) was cloned and sequenced. Complete genome comparisons indicated that ONMV, currently classified as a rymovirus, was most closely related ( approximately 73% nucleotide, approximately 79% amino acid identity) to the tritimovirus Wheat streak mosaic virus (WSMV). ONMV encoded a single polyprotein, with proteinase cleavage sites very similar to those of WSMV. Pairwise comparison of ONMV and WSMV cistrons revealed that P3 was most conserved ( approximately 79% nucleotide, approximately 86% amino acid), whereas HC-Pro was most divergent ( approximately 67% nucleotide, approximately 69% amino acid). In contrast, the ONMV sequence was distantly related ( approximately 40% nt, approximately 26% amino acid identity) to that of the rymoviru s Ryegrass mosaic virus, with highest sequence conservation noted within the NIb cistron ( approximately 47% nucleotide, approximately 41% amino acid identity). These results firmly establish that ONMV is not a rymovirus but is instead a distinct species of the genus Tritimovirus.
燕麦坏死斑驳病毒（ONMV）的RNA基因组（9346个核苷酸）被克隆并测序。全基因组比较表明，目前被归类为黑麦草花叶病毒属的ONMV与小麦线条花叶病毒（WSMV，小麦花叶病毒属）关系最为密切（核苷酸相似度约为73%，氨基酸相似度约为79%）。ONMV编码一种单一的多蛋白，其蛋白酶切割位点与WSMV的非常相似。ONMV和WSMV顺反子的成对比较显示，P3最为保守（核苷酸约79%，氨基酸约86%），而HC-Pro差异最大（核苷酸约67%，氨基酸约69%）。相比之下，ONMV序列与黑麦草花叶病毒属的黑麦草花叶病毒关系较远（核苷酸相似度约40%，氨基酸相似度约26%），在NIb顺反子内序列保守性最高（核苷酸约47%，氨基酸约41%）。这些结果明确证实，ONMV不是黑麦草花叶病毒属病毒，而是小麦花叶病毒属的一个独特种。
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