高加索薯蓣中寡呋喃甾苷及其特异性降解β-葡萄糖苷酶的组织和亚细胞定位

Tissue and subcellular localization of oligofurostanosides and their specific degrading beta-glucosidase in Dioscorea caucasica Lipsky.

作者信息

Gurielidze Koba, Gogoberidze Mzevinar, Dadeshidze Inga, Vardosanidze Maia, Djaoshvili Maia, Lomkatsi Nino

机构信息

Durmischidze Institute of Biochemistry and Biotechnology, Department of Cell Culture and Biologically Active Compounds, Tbilisi 0159, Georgia.

出版信息

Phytochemistry. 2004 Mar;65(5):555-9. doi: 10.1016/j.phytochem.2003.12.016.

DOI:10.1016/j.phytochem.2003.12.016

PMID:15003418

Abstract

The application of the histochemical technique using the Ehrlich reagent showed that the oligofurostanosides--the steroid glycosides of the furostan series--are localized in idioblasts (special cells--receptacles) of the epidermal layer of Dioscorea caucasica leaves. The activity of oligofurostanoside-specific beta-glucosidase was localized mainly in the membrane fraction of the thylakoides and was also found in the Dioscorea caucasica leaves. We have suggested, that the differential tissue, the subcellular localization of the oligofurostanosides and their degrading enzymes provide the maintenance of the furostanol structure of steroid glycosides which is necessary for their transport within the leaf and from the leaf to the rhizome.

摘要

使用埃利希试剂的组织化学技术应用表明，寡呋甾烷苷——呋甾烷系列的甾体糖苷——定位于高加索薯蓣叶片表皮层的异细胞（特殊细胞——容器）中。寡呋甾烷苷特异性β-葡萄糖苷酶的活性主要定位于类囊体的膜部分，在高加索薯蓣叶片中也有发现。我们认为，寡呋甾烷苷及其降解酶在不同组织和亚细胞水平的定位有助于维持甾体糖苷的呋甾醇结构，这对于它们在叶片内以及从叶片到根茎的运输是必要的。