Temma Kumiko, Shimoya Koichiro, Hashimoto Kazumasa, Zhang Qing, Koyama Masayasu, Murata Yuji
Department of Obstetrics and Gynecology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita City, Osaka 565-0871, Japan.
Fertil Steril. 2004 Mar;81 Suppl 1:798-801. doi: 10.1016/j.fertnstert.2003.07.039.
To examine the presence of erythropoietin (EPO) in ejaculates and the association between EPO levels in seminal plasma and semen parameters.
Retrospective analysis.
University hospital in Japan.
PATIENT(S): Eighty-three infertile males and 16 volunteers with proven fertility.
INTERVENTION(S): Semen was obtained by masturbation after 5 days of abstinence. Blood sample and split ejaculates of 16 volunteers with proven fertility were collected.
MAIN OUTCOME MEASURE(S): Western blot analysis and enzyme-linked immunoassay in the seminal plasma and sperm parameters.
RESULT(S): Western blot analysis showed that EPO protein was present in the seminal plasma. The EPO titers in the seminal plasma ranged from 1.5 mIU/mL to 45.0 mIU/mL by enzyme-linked immunoassay. There was no significant association between EPO levels and semen parameters. The first fraction of samples obtained by split ejaculation contained almost the same amount of EPO as the second fraction.
CONCLUSION(S): Erythropoietin protein was constitutively present in seminal plasma. The seminal EPO originated from the prostate and seminal vesicle. No association between EPO levels in seminal plasma and sperm parameters was found in the present study.
检测射精液中促红细胞生成素（EPO）的存在情况以及精浆中EPO水平与精液参数之间的关联。
回顾性分析。
日本大学医院。
83名不育男性和16名已证实有生育能力的志愿者。
禁欲5天后通过手淫获取精液。收集16名已证实有生育能力的志愿者的血液样本和分段射精液。
精浆的蛋白质免疫印迹分析和酶联免疫吸附测定以及精子参数。
蛋白质免疫印迹分析显示精浆中存在EPO蛋白。通过酶联免疫吸附测定，精浆中的EPO滴度范围为1.5 mIU/mL至45.0 mIU/mL。EPO水平与精液参数之间无显著关联。分段射精获得的样本的第一部分所含EPO量与第二部分几乎相同。
促红细胞生成素蛋白在精浆中持续存在。精浆中的EPO源自前列腺和精囊。本研究未发现精浆中EPO水平与精子参数之间存在关联。
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