在二态接合菌卷枝毛霉中发现的一种新型真菌异戊烯基二磷酸合酶。

A novel fungal prenyl diphosphate synthase in the dimorphic zygomycete Mucor circinelloides.

作者信息

Velayos Antonio, Fuentes-Vicente Mónica, Aguilar-Elena Raúl, Eslava Arturo P, Iturriaga Enrique A

机构信息

Area de Genética, Departamento de Microbiología y Genética, Universidad de Salamanca, Edificio Departamental, Lab 301-2, Avenida Campo Charro s/n, 37007 Salamanca, Spain.

出版信息

Curr Genet. 2004 Jun;45(6):371-7. doi: 10.1007/s00294-004-0498-4. Epub 2004 Mar 13.

DOI:10.1007/s00294-004-0498-4

PMID:15024605

Abstract

Two Mucor circinelloides structural genes involved in isoprenoid biosynthesis were isolated and characterised. The isoA gene encodes a typical eukaryotic farnesyl diphosphate synthase (EC 2.5.1.10), whereas the isoB gene deduced amino acid sequence shows similarity to fungal medium-chain prenyl diphosphate synthases. By functional complementation in Escherichia coli, the isoB gene product was shown to be a solanesyl diphosphate synthase (EC 2.5.1.11), which is the first fungal enzyme reported having this specificity. In addition, a M. circinelloides one-marker-per-chromosome map was completed by contour-clamped homogeneous electric field localisation of isoA, isoB and three other isoprenoid biosynthesis genes to individual chromosomes.

摘要

分离并鉴定了参与类异戊二烯生物合成的两个卷枝毛霉结构基因。isoA基因编码一种典型的真核法呢基二磷酸合酶（EC 2.5.1.10），而isoB基因推导的氨基酸序列与真菌中链异戊烯基二磷酸合酶具有相似性。通过在大肠杆菌中的功能互补，isoB基因产物被证明是一种茄呢基二磷酸合酶（EC 2.5.1.11），这是报道的第一种具有这种特异性的真菌酶。此外，通过将isoA、isoB和其他三个类异戊二烯生物合成基因进行轮廓钳位均匀电场定位到单个染色体上，完成了卷枝毛霉每条染色体一个标记的图谱。