Detection of multiple beta-casein (CASB) alleles by amplification created restriction sites (ACRS).
作者信息
Lien S, Aleström P, Klungland H, Rogne S
机构信息
Department of Animal Science, Agricultural University, As, Norway.
出版信息
Anim Genet. 1992;23(4):333-8. doi: 10.1111/j.1365-2052.1992.tb00155.x.
Abstract
Direct sequencing of polymerase chain reaction (PCR) amplified DNA has been used to detect the DNA sequences for bovine beta-casein (CASB) A3 and B variants. Based on these sequences we have designed primers which create allele-specific restriction sites in the PCR product. Restriction analysis of PCR product generated in one reaction enable us to identify the A1, A2, A3 and B alleles of CASB rapidly without the use of radioactivity.
摘要
Zotero 插件