通过彗星试验评估野生嘉赐树提取物对HTC和V79细胞的遗传毒性潜力。
Evaluation of the genotoxic potential of the Casearia sylvestris extract on HTC and V79 cells by the comet assay.
作者信息
Maistro E L, Carvalho J C T, Mantovani M S
机构信息
Instituto de Farmácia e Nutrição, UNIFENAS, 37130-000, Alfenas, Minas Gerais, Brazil.
出版信息
Toxicol In Vitro. 2004 Jun;18(3):337-42. doi: 10.1016/j.tiv.2003.10.002.
Casearia sylvestris is common in tropical America growing wild in Brazil in the states of Amazonas and São Paulo. Its leaves are used in Brazilian folk medicine for several diseases. The present investigation was carried out to examine the genotoxic effects of a C. sylvestris crude ethanolic extract on Hepatoma Tissue Culture (HTC cells) of Rattus norvegicus and Chinese hamster V79 cells in culture, using the comet assay. For the genotoxic evaluation the cells were treated with three different concentrations (0.5, 1 and 2 mg/ml) of extract prepared from a 25 mg/ml aqueous solution. The positive control was cyclophosphamide for HTC cells and methyl methanesulfonate for V79 cells. The duration of the treatment was 2 h. The results showed that the extract of C. sylvestris presented no genotoxic effects and not modified effect inducing DNA damage by alkylating agents cyclophosphamide and methyl methanesulfonate in HTC and V 79 cells respectively.
西印度格木在热带美洲很常见，在巴西的亚马孙州和圣保罗州野生生长。其叶子在巴西民间医学中用于治疗多种疾病。本研究旨在使用彗星试验检测西印度格木粗乙醇提取物对挪威大鼠肝癌组织培养细胞（HTC细胞）和中国仓鼠V79细胞的遗传毒性作用。为了进行遗传毒性评估，用从25mg/ml水溶液制备的三种不同浓度（0.5、1和2mg/ml）的提取物处理细胞。HTC细胞的阳性对照是环磷酰胺，V79细胞的阳性对照是甲基磺酸甲酯。处理持续时间为2小时。结果表明，西印度格木提取物没有遗传毒性作用，并且分别对HTC和V79细胞中通过烷基化剂环磷酰胺和甲基磺酸甲酯诱导DNA损伤没有修饰作用。
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