Van Loock M, Lambin S, Volckaert G, Goddeeris B M, Vanrompay D
Department of Animal Sciences, Catholic University of Leuven, Kasteelpark Arenberg 30, 3001 Leuven, Belgium.
Vaccine. 2004 Apr 16;22(13-14):1616-23. doi: 10.1016/j.vaccine.2003.10.044.
Plasmid DNA (pcDNA1::MOMP D) expressing the major outer membrane protein (MOMP) of an avian Chlamydophila psittaci serovar D strain was tested for its ability to induce protective immunity against C. psittaci challenge in the presence of maternal antibodies. A combined parenteral (intramuscular injection) and mucosal route (DNA drops administered to the nares) of DNA inoculation was used. Following pcDNA1::MOMP vaccination, both T helper and B cell memory were primed. However, high maternal antibodies titres affected the induction of vaccine-specific antibody responses as assessed by MOMP-specific antibody levels in enzyme-linked immunosorbent assay (ELISA). Cell-mediated immunity was unaltered as demonstrated by the significantly heightened proliferative responses of peripheral blood lymphocytes (PBL) following vaccination. DNA vaccination could significantly reduce clinical symptoms, pharyngeal and cloacal excretion as well as Chlamydophila replication, even in the presence of maternal antibodies.
对表达禽鹦鹉热衣原体D血清型菌株主要外膜蛋白（MOMP）的质粒DNA（pcDNA1::MOMP D）进行了测试，以评估其在母源抗体存在的情况下诱导针对鹦鹉热衣原体攻击的保护性免疫的能力。采用了联合的肠胃外（肌肉注射）和黏膜途径（经鼻滴注DNA）进行DNA接种。接种pcDNA1::MOMP疫苗后，辅助性T细胞和B细胞记忆均被启动。然而，通过酶联免疫吸附测定（ELISA）中的MOMP特异性抗体水平评估，高母源抗体滴度影响了疫苗特异性抗体反应的诱导。如接种疫苗后外周血淋巴细胞（PBL）增殖反应显著增强所示，细胞介导的免疫未改变。即使在存在母源抗体的情况下，DNA疫苗接种也能显著减轻临床症状、咽部和泄殖腔排泄物以及鹦鹉热衣原体的复制。
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