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用于诊断与致泻性大肠杆菌相关的肠道感染的多重聚合酶链反应

Multiplex PCR for diagnosis of enteric infections associated with diarrheagenic Escherichia coli.

作者信息

Vidal Roberto, Vidal Maricel, Lagos Rossana, Levine Myron, Prado Valeria

机构信息

Programa de Microbiologia, Institode Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, Santiaago.

出版信息

J Clin Microbiol. 2004 Apr;42(4):1787-9. doi: 10.1128/JCM.42.4.1787-1789.2004.

Abstract

A multiplex PCR for detection of three categories of diarrheagenic Escherichia coli was developed. With this method, enterohemorrhagic E. coli, enteropathogenic E. coli, and enterotoxigenic E. coli were identified in fecal samples from patients with hemorrhagic colitis, watery diarrhea, or hemolytic-uremic syndrome and from food-borne outbreaks.

摘要

开发了一种用于检测三类致泻性大肠杆菌的多重聚合酶链反应。通过这种方法,在出血性结肠炎、水样腹泻或溶血尿毒综合征患者的粪便样本以及食源性疾病暴发中,鉴定出了肠出血性大肠杆菌、肠致病性大肠杆菌和产肠毒素大肠杆菌。

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