来自枯草芽孢杆菌噬菌体phi 29核蛋白的核肽片段H-Asp-Ser[pAAAGTAAGCC]-Glu-OH的固相合成
Solid-phase synthesis of the nucleopeptide fragment H-Asp-Ser[pAAAGTAAGCC]-Glu-OH from the nucleoprotein of Bacillus subtilis phage phi 29.
作者信息
Dreef-Tromp C M, van der Maarel J C, van den Elst H, van der Marel G A, van Boom J H
机构信息
Gorlaeus Laboratories, Leiden, The Netherlands.
出版信息
Nucleic Acids Res. 1992 Aug 11;20(15):4015-20. doi: 10.1093/nar/20.15.4015.
Abstract
The naturally occurring DNA-nucleopeptide H-Asp-Ser[5'-pAAAGTAAGCC-3']-Glu-OH was prepared via a solid-phase phosphite triester approach using N-2-(tert-butyldiphenylsilyloxymethyl)benzoyl protected nucleosides. The oligonucleotide was linked via the extremely base-labile oxalyl ester anchor to the solid support.
摘要
天然存在的DNA-核苷肽H-Asp-Ser[5'-pAAAGTAAGCC-3']-Glu-OH是通过使用N-2-(叔丁基二苯基硅氧基甲基)苯甲酰基保护的核苷，采用固相亚磷酸三酯法制备的。寡核苷酸通过对碱基极其不稳定的草酰酯锚定连接到固相载体上。
相似文献
1
Solid-phase synthesis of the nucleopeptide fragment H-Asp-Ser[pAAAGTAAGCC]-Glu-OH from the nucleoprotein of Bacillus subtilis phage phi 29.来自枯草芽孢杆菌噬菌体phi 29核蛋白的核肽片段H-Asp-Ser[pAAAGTAAGCC]-Glu-OH的固相合成
Nucleic Acids Res. 1992 Aug 11;20(15):4015-20. doi: 10.1093/nar/20.15.4015.
2
Solid-phase synthesis of an RNA nucleopeptide fragment from the nucleoprotein of poliovirus.脊髓灰质炎病毒核蛋白RNA核肽片段的固相合成。
Nucleic Acids Res. 1992 May 25;20(10):2435-9. doi: 10.1093/nar/20.10.2435.
3
Solid-phase synthesis of H-Phe-Tyr-(pATAT)-NH2: a nucleopeptide fragment from the nucleoprotein of bacteriophage phi X174.H-Phe-Tyr-(pATAT)-NH2的固相合成：来自噬菌体phi X174核蛋白的一个核肽片段。
Nucleic Acids Res. 1990 Nov 25;18(22):6491-5. doi: 10.1093/nar/18.22.6491.
4
Template requirements for initiation of phage phi 29 DNA replication in vitro.体外启动噬菌体φ29 DNA复制的模板要求。
Proc Natl Acad Sci U S A. 1984 Jan;81(1):80-4. doi: 10.1073/pnas.81.1.80.
5
High level synthesis in Escherichia coli of the Bacillus subtilis phage phi 29 proteins p3 and p4 under the control of phage lambda PL promoter.在噬菌体λ PL 启动子的控制下，枯草芽孢杆菌噬菌体 phi 29 蛋白 p3 和 p4 在大肠杆菌中的高水平合成。
Nucleic Acids Res. 1982 Oct 11;10(19):5773-84. doi: 10.1093/nar/10.19.5773.
6
Solid-phase synthesis of a nucleopeptide from the linking site of adenovirus-2 nucleoprotein, -Ser(p5'CATCAT)-Gly-Asp-. Convergent versus stepwise strategy.从腺病毒2型核蛋白的连接位点-Ser(p5'CATCAT)-Gly-Asp-固相合成核肽。汇聚法与逐步合成法策略
Nucleic Acids Res. 1995 Oct 25;23(20):4151-61. doi: 10.1093/nar/23.20.4151.
7
Nucleotide sequence of the early genes 3 and 4 of bacteriophage phi 29.噬菌体φ29早期基因3和4的核苷酸序列。
Nucleic Acids Res. 1982 Oct 11;10(19):5785-98. doi: 10.1093/nar/10.19.5785.
8
[Construction of phage vector for Bacillus subtilis].[枯草芽孢杆菌噬菌体载体的构建]
Wei Sheng Wu Xue Bao. 1991 Oct;31(5):376-83.
9
Location of the serine residue involved in the linkage between the terminal protein and the DNA of phage phi 29.
Nucleic Acids Res. 1985 Nov 11;13(21):7715-28. doi: 10.1093/nar/13.21.7715.
10
Cloning of the Bacillus subtilis spoIIA and spoV A loci in phage phi 105DI:1t.枯草芽孢杆菌spoIIA和spoVA基因座在噬菌体phi 105DI:1t中的克隆。
J Gen Microbiol. 1984 Aug;130(8):2137-45. doi: 10.1099/00221287-130-8-2137.
引用本文的文献
1
Studies on the synthesis of nucleotidyl-peptides. II. The preparation of a nucleotidyl-peptide having a 5'-nucleotidyl-(P-O)-serine phosphodiester bond.核苷酸肽的合成研究。II. 具有5'-核苷酸-(P-O)-丝氨酸磷酸二酯键的核苷酸肽的制备。
Nucleic Acids Res. 1993 Sep 25;21(19):4451-7. doi: 10.1093/nar/21.19.4451.
2
Solid-phase synthesis of a nucleopeptide from the linking site of adenovirus-2 nucleoprotein, -Ser(p5'CATCAT)-Gly-Asp-. Convergent versus stepwise strategy.从腺病毒2型核蛋白的连接位点-Ser(p5'CATCAT)-Gly-Asp-固相合成核肽。汇聚法与逐步合成法策略
Nucleic Acids Res. 1995 Oct 25;23(20):4151-61. doi: 10.1093/nar/23.20.4151.
本文引用的文献
1
Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support.寡脱氧核糖核苷酸的快速合成。VII. 在硅藻土-聚酰胺载体上通过连续流动磷酸三酯法固相合成寡脱氧核糖核苷酸。
Nucleic Acids Res. 1982 Oct 25;10(20):6243-54. doi: 10.1093/nar/10.20.6243.
2
Protein p3 is linked to the DNA of phage phi 29 through a phosphoester bond between serine and 5'-dAMP.蛋白质p3通过丝氨酸与5'-二磷酸腺苷之间的磷酸酯键与噬菌体φ29的DNA相连。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6425-8. doi: 10.1073/pnas.77.11.6425.
3
Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.聚合物载体寡核苷酸合成 XVIII：脱氧核苷的β-氰基乙基-N，N-二烷基氨基-/N-吗啉代亚磷酰胺用于 DNA 片段合成，简化最终产物的脱保护和分离
Nucleic Acids Res. 1984 Jun 11;12(11):4539-57. doi: 10.1093/nar/12.11.4539.
4
Synthetic nucleotide-peptides.合成核苷酸肽
Prog Nucleic Acid Res Mol Biol. 1970;10:145-82. doi: 10.1016/s0079-6603(08)60564-4.
5
Location of the serine residue involved in the linkage between the terminal protein and the DNA of phage phi 29.
Nucleic Acids Res. 1985 Nov 11;13(21):7715-28. doi: 10.1093/nar/13.21.7715.
6
A model study directed towards the preparation of nucleopeptides via H-phosphonate intermediates.一项旨在通过H-膦酸酯中间体制备核肽的模型研究。
Nucleic Acids Res. 1987 Feb 25;15(4):1807-18. doi: 10.1093/nar/15.4.1807.
7
Initiation of viral DNA replication.
Adv Virus Res. 1988;34:1-42. doi: 10.1016/s0065-3527(08)60514-x.
8
Synthesis of the nucleopeptides H-Phe-Tyr(pGC)-NH2 and H-Phe-Ser(pGC)-Ala-OH via a phosphotriester approach.通过磷酸三酯法合成核肽H-Phe-Tyr(pGC)-NH2和H-Phe-Ser(pGC)-Ala-OH。
Nucleic Acids Res. 1989 Apr 25;17(8):2897-905. doi: 10.1093/nar/17.8.2897.
9
Solid-phase synthesis of H-Phe-Tyr-(pATAT)-NH2: a nucleopeptide fragment from the nucleoprotein of bacteriophage phi X174.H-Phe-Tyr-(pATAT)-NH2的固相合成：来自噬菌体phi X174核蛋白的一个核肽片段。
Nucleic Acids Res. 1990 Nov 25;18(22):6491-5. doi: 10.1093/nar/18.22.6491.
10
Oxalyl-CPG: a labile support for synthesis of sensitive oligonucleotide derivatives.草酰基-CPG：用于合成敏感寡核苷酸衍生物的不稳定载体。
Nucleic Acids Res. 1991 Apr 11;19(7):1527-32. doi: 10.1093/nar/19.7.1527.
Zotero 插件