Mania-Pramanik Jayanti, Potdar Shobha S, Vadigoppula Ashok, Sawant Shankar
National Institute for Research in Reproductive Health, Indian Council of Medical Research, Mumbai, India.
J Clin Lab Anal. 2004;18(3):153-8. doi: 10.1002/jcla.20015.
The present study was undertaken to estimate elastase in biological fluids and assess its usefulness as an indicator of inflammation/infection. Elastase was measured in seminal plasma, serum, urine, and cervical specimens using a specific substrate and was expressed in arbitrary units (AU). It was found to be stable over a period of 3 weeks. The intra- and interassay variation of elastase assay was between 2.3 to 6.8% and 7.3 to 9.9%, respectively. The assay was validated by comparing it with other methods that are available for the detection of infections. Sensitivity of the assay indicating inflammation/infection in these samples varied between 70.9 to 87.3%. The results obtained suggest that the presence of elastase in specimens may be used as a nonspecific indicator and could be used to screen inflammation/infection in a limited resource setting.
本研究旨在测定生物体液中的弹性蛋白酶,并评估其作为炎症/感染指标的效用。使用特定底物测定精浆、血清、尿液和宫颈标本中的弹性蛋白酶,并以任意单位(AU)表示。结果发现其在3周内保持稳定。弹性蛋白酶测定的批内和批间变异分别在2.3%至6.8%和7.3%至9.9%之间。通过与其他可用于检测感染的方法进行比较,对该测定方法进行了验证。该测定方法在这些样本中指示炎症/感染的敏感性在70.9%至87.3%之间。所得结果表明,标本中弹性蛋白酶的存在可作为一种非特异性指标,可用于在资源有限的情况下筛查炎症/感染。