通过流动注射分析法连续测定药物中的硫胺素和抗坏血酸。

Successive determination of thiamine and ascorbic acid in pharmaceuticals by flow injection analysis.

作者信息

Pérez-Ruiz Tomás, Martínez-Lozano Carmen, Sanz Antonio, Guillén Ana

机构信息

Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia, Spain.

出版信息

J Pharm Biomed Anal. 2004 Feb 18;34(3):551-7. doi: 10.1016/s0731-7085(03)00606-x.

DOI:10.1016/s0731-7085(03)00606-x

PMID:15127811

Abstract

A simple and rapid fluorimetric method for the determination of mixtures of thiamine and ascorbic acid is proposed. The procedure is based on the oxidation with mercury(II) of the B1 and C vitamins to form thiochrome (TC) and quinoxaline derivate, respectively. Both reaction products exhibit fluorescence at the same wavelengths (lambdaex = 356 and lambdaem = 440 nm). The procedure is optimised in a flow injection (FI) system and applied with excellent results in the determination of B1 and C vitamins in commercial pharmaceutical preparations. The calibration graphs were linear over the range 2-100 microg ml(-1) for thiamine and 5-100 microg ml(-1) for ascorbic acid. The throughput was 25 samples per hour.

摘要

提出了一种简单快速的荧光法测定硫胺素和抗坏血酸混合物。该方法基于用汞（II）分别氧化维生素B1和维生素C，形成硫色素（TC）和喹喔啉衍生物。两种反应产物在相同波长（激发波长λex = 356，发射波长λem = 440 nm）下均显示荧光。该方法在流动注射（FI）系统中进行了优化，并成功应用于商业药物制剂中维生素B1和维生素C的测定。硫胺素的校准曲线在2 - 100 μg ml⁻¹范围内呈线性，抗坏血酸的校准曲线在5 - 100 μg ml⁻¹范围内呈线性。每小时可处理25个样品。