中华人民共和国湖南省沅江地区田鼠（东方田鼠）中弓形虫的血清学流行情况。

Serologic prevalence of Toxoplasma gondii in field mice, Microtus fortis, from Yuanjiang, Hunan Province, People's Republic of China.

作者信息

Zhang S Y, Jiang S F, He Y Y, Pan C E, Zhu M, Wei M X

机构信息

Department of Parasitic Diseases, Shanghai Municipal Center for Diseases Prevention and Control, Shanghai 200336, People's Republic of China.

出版信息

J Parasitol. 2004 Apr;90(2):437-8. doi: 10.1645/GE-168R.

DOI:10.1645/GE-168R

PMID:15165081

Abstract

Antibodies to Toxoplasma gondii were investigated in serum samples of field mice, Microtus fortis, from Yuanjiang, Hunan Province, People's Republic of China. The modified agglutination test (MAT) incorporating formalin-fixed whole tachyzoites and mercaptoethanol was used to determine antibodies. Antibodies to T. gondii (MAT > or = 1:20) were found in 36 (29%) of 124 trapped mice. The antibody titers of positive sera (percentage in parentheses) were 1:20 (8.9), 1:40 (3.2), 1:80 (3.2), 1:160 (1.6), 1:320 (1.6), 1:640 (1.6), 1:1,280 (1.6), 1: 2,560 (0.8), and > 1:2,560 (6.5). No antibody to T. gondii was found in 104 sera of laboratory-bred M. fortis infected with Schistosoma japonicum between 1 and 45 days after infection.

摘要

在中国湖南省沅江捕获的东方田鼠血清样本中检测了抗刚地弓形虫抗体。采用改良凝集试验（MAT），以甲醛固定的全速殖子和巯基乙醇来检测抗体。在124只捕获的小鼠中，有36只（29%）检测到抗刚地弓形虫抗体（MAT≥1:20）。阳性血清的抗体滴度（括号内为百分比）分别为1:20（8.9%）、1:40（3.2%）、1:80（3.2%）、1:160（1.6%）、1:320（1.6%）、1:640（1.6%）、1:1280（1.6%）、1:2560（0.8%）和＞1:2560（6.5%）。在感染日本血吸虫1至45天的104份实验室饲养的东方田鼠血清中未检测到抗刚地弓形虫抗体。