Effect of binding and conformation on fluorescence quenching in new 2',7'-dichlorofluorescein derivatives.
作者信息
Sparano Brian A, Shahi Shatrughan P, Koide Kazunori
机构信息
Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, Pennsylvania 15260, USA.
出版信息
Org Lett. 2004 Jun 10;6(12):1947-9. doi: 10.1021/ol049537y.
PMID:15176790
Abstract
[structure: see text] Symmetrical and unsymmetrical 2',7'-dichlorofluorescein (DCF) derivatives have been synthesized by means of Mannich reactions and an aromatic Claisen rearrangement. NMR and fluorescence spectroscopic studies reveal the correlation between the conformations, the photoinduced electron transfer mechanism, and fluorescent intensities of these DCF derivatives. Two quenching nitrogen atoms cooperatively and reversibly suppress the fluorescence of the chromophore.
摘要
Zotero 插件