吲哚菁绿在脂肪乳剂溶液中的发射和吸收特性。
Emission and absorption properties of indocyanine green in Intralipid solution.
作者信息
Yuan Baohong, Chen NanGuang, Zhu Quing
机构信息
Department of Electrical and Computer Engineering, University of Connecticut, Storrs, CT 06269-1157, USA.
出版信息
J Biomed Opt. 2004 May-Jun;9(3):497-503. doi: 10.1117/1.1695411.
Emission and absorption properties of indocyanine green (ICG) in Intralipid solution have been investigated. The study is focused on relatively low ICG concentration at a range of 0 to 20 microM. A diffusion model was used to analyze the emission properties of ICG solution at different concentrations. In the low-concentration region, the emission strength increases with the concentration of ICG, while in the high-concentration region, the emission decreases with the concentration. In general, a maximum of emission strength exists and its position (concentration) depends on the wavelength of the excitation light, the distance between the source and the detector, and the sample geometry and size. A so-called "inner-cell-effect" and re-absorption of emission photons are found to contribute to the decay of emission strength. Also, in the concentration range of 0 to 2 microM, ICG solution always has a higher absorption coefficient at wavelength 830 nm than that at 660 nm, which is quite different from the ICG in water case.
研究了吲哚菁绿（ICG）在脂质乳剂溶液中的发射和吸收特性。该研究聚焦于0至20微摩尔范围内相对较低的ICG浓度。使用扩散模型分析不同浓度下ICG溶液的发射特性。在低浓度区域，发射强度随ICG浓度增加而增加，而在高浓度区域，发射随浓度降低。一般来说，存在发射强度的最大值，其位置（浓度）取决于激发光的波长、光源与探测器之间的距离以及样品的几何形状和尺寸。发现所谓的“细胞内效应”和发射光子的再吸收会导致发射强度的衰减。此外，在0至2微摩尔的浓度范围内，ICG溶液在830纳米波长处的吸收系数始终高于660纳米波长处，这与ICG在水中的情况有很大不同。
Zotero 插件