[Using the FRTL-5 cell to screen the thyroxine disrupting effects of the two pesticides-ethylenethiourea and pendimethalin].
作者信息
Pan Hongmei, Sun Ding, Wang Jintao, Wu Desheng
机构信息
Huaxi School of Public Health, Sichuan University, Chengdu 610041, China.
出版信息
Wei Sheng Yan Jiu. 2004 May;33(3):267-9.
OBJECTIVE
In order to study the effects of ethylenethiourea(ETU) and pendimethalin on synthesis and secretion of thyroglobulin(Tg) in FRTL-5 cells in vitro.
METHODS
MTT test was used to investigate the cytotoxicity of the two chemicals to FRTL-5 cell. When the concentration of pendimethalin exceeded 5 ng/microliter, and the concentration of ethylenethiourea exceeded 360 ng/microliter, there was distinct cytotoxicity to FRTL-5 cell. The radioimmunoassay was also used to detect the level of thyroglobulin in culture. The exposure doses in radioimmunoassay was based on the MTT test.
RESULTS
The concentration of Tg was decreased in medium and high dose of pendimethalin and ethylenethiourea.
CONCLUSION
It is likely, therefore, that the two pesticides might disrupt FRTL-5 cell of synthesizing and secreting Tg.
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