SNPbox：基于网络的从基因到基因组的高通量引物设计
SNPbox: web-based high-throughput primer design from gene to genome.
作者信息
Weckx Stefan, De Rijk Peter, Van Broeckhoven Christine, Del-Favero Jurgen
机构信息
Department of Molecular Genetics, Bioinformatics Unit, Flanders Interuniversity Institute for Biotechnology, University of Antwerp, Belgium.
出版信息
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W170-2. doi: 10.1093/nar/gkh369.
Abstract
SNPbox is a modular software package that automates the design of PCR primers for large-scale amplification and sequencing projects in a standardized manner resulting in high-quality PCR amplicons with a low failure rate. Here, we present the SNPbox web server at http://www.SNPbox.org, which hosts the SNPbox web service as well as the data from SNPbox analysis of all Ensembl exons. The data of this genome-wide SNPbox application can be visualized in Ensembl's ContigView through a DAS (distributed annotation system) annotation server.
摘要
SNPbox是一个模块化软件包，它以标准化方式自动设计用于大规模扩增和测序项目的PCR引物，从而产生高质量且失败率低的PCR扩增子。在此，我们展示了位于http://www.SNPbox.org的SNPbox网络服务器，它承载着SNPbox网络服务以及所有Ensembl外显子的SNPbox分析数据。这个全基因组SNPbox应用的数据可以通过一个分布式注释系统（DAS）注释服务器在Ensembl的ContigView中可视化。
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