Developmental changes of Ni(2+) sensitivity and automaticity in Nkx2.5-positive cardiac precursor cells from murine embryonic stem cell.
作者信息
Manabe Kasumi, Miake Junichiro, Sasaki Norihito, Furuichi Hitomi, Yano Shuichi, Mizuta Einosuke, Yamamoto Yasutaka, Hoshikawa Yoshiko, Yamazaki Hidetoshi, Tajima Fumihito, Shiota Goshi, Nanba Eiji, Ohgi Shigetsugu, Hidaka Kyoko, Morisaki Takayuki, Kurata Yasutaka, Lee Jong-Kook, Igawa Osamu, Shigemasa Chiaki, Hisatome Ichiro
机构信息
Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science, Tottori, Japan.
出版信息
Circ J. 2004 Jul;68(7):724-6. doi: 10.1253/circj.68.724.
BACKGROUND
It is controversial which subtypes of T type Ca(2+) channels are implicated in automaticity of cardiac cells during the embryonic period.
METHOD AND RESULTS
The effect of Ni(2+) on the automaticity of Nkx2.5-positive cardiac precursor cells sorted from embryonic stem cells during their differentiation was examined using patch clamp techniques. Although 40 micromol/L Ni(2+), which is enough to block Ni(2+)sensitive T type-Ca(2+) channels, decreased the spontaneous beating rate in all cells in the early and intermediate stage, Ni(2+) did not show any effects on the automaticity of 50% of the cells in the late stage.
CONCLUSION
These results indicate that Ni(2+)-sensitive T-type Ca(2+) channels expressed in the Nkx2.5-positive cardiac precursor cells are developmentally regulated.
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