Wu De-Hua, Liu Li, Chen Long-Hua, Ding Yan-Qing
Department of Radiation Oncology, First Military Medical University, Guangzhou 510515, Guangdong Province, China.
World J Gastroenterol. 2004 Aug 1;10(15):2245-9. doi: 10.3748/wjg.v10.i15.2245.
To investigate KAI1 gene expression in the progression of human colonic carcinoma and its clinical significances.
KAI1 expression was detected by in situ hybridization and immunohistochemistry in the 4 established cell lines of colorectal carcinoma with different metastatic potentials, and in 80 specimens of colonic carcinoma, 21 colonic carcinoma specimens with lymphatic metastasis and 20 controls of normal colonic mucosa.
The expressions of KAI1 in HT29 and SW480 cell lines were higher than those in LoVo and SW620. The expression of KAI1 gene was significantly higher in colorectal carcinoma compared with normal colonic mucosa and lymphatic metastasis (chi(2)=46.838, P<0.01). The expression of KAI1 gene had no relationship with histological grade. The KAI1 expressions in Dukes A and B carcinoma were higher at both mRNA and protein levels compared to Dukes C carcinoma (chi(2)=16.061, P<0.05). The expression of KAI1 in colonic carcinoma specimens with lymphatic metastasis was almost lost. The results of in situ hybridization were in concordance with immunohistochemistry.
KAI1 is highly related to the metastasis of colonic carcinoma and may be a useful indicator of metastasis in colonic carcinoma.
探讨KAI1基因在人结肠癌进展中的表达及其临床意义。
采用原位杂交和免疫组化方法检测4种具有不同转移潜能的结直肠癌 established 细胞系、80例结肠癌标本、21例有淋巴结转移的结肠癌标本及20例正常结肠黏膜对照中KAI1的表达。
HT29和SW480细胞系中KAI1的表达高于LoVo和SW620。与正常结肠黏膜和淋巴结转移相比,KAI1基因在结直肠癌中的表达明显更高(χ²=46.838,P<0.01)。KAI1基因的表达与组织学分级无关。与Dukes C期癌相比,Dukes A和B期癌中KAI1在mRNA和蛋白水平的表达均更高(χ²=16.061,P<0.05)。有淋巴结转移的结肠癌标本中KAI1的表达几乎消失。原位杂交结果与免疫组化结果一致。
KAI1与结肠癌转移高度相关,可能是结肠癌转移的一个有用指标。