Tollefsbol Trygve O
Department of Biology, University of Alabama at Birmingham, USA.
Methods Mol Biol. 2004;287:1-8. doi: 10.1385/1-59259-828-5:001.
Epigenetics encompasses heritable changes in DNA or its associated proteins except mutations in gene sequence. Many investigators in the field of epigenetics focus on histone modifications and DNA methylation, two molecular mechanisms that are often linked and interdependent. A variety of methods are applied to the study of epigenetic processes, and the past decade has witnessed an exponential increase in novel approaches to elucidate the molecular mysteries of epigenetic inheritance. This chapter summarizes some of the most contemporary methods used to study epigenetics presented throughout the book.
表观遗传学涵盖了DNA或其相关蛋白质中除基因序列突变以外的可遗传变化。表观遗传学领域的许多研究人员专注于组蛋白修饰和DNA甲基化,这两种分子机制通常相互关联且相互依存。多种方法被应用于表观遗传过程的研究,在过去十年中,用于阐明表观遗传遗传分子奥秘的新方法呈指数级增长。本章总结了全书介绍的一些研究表观遗传学的最现代方法。