地中海拟无枝酸菌DSM 46095重新分类为利福霉素拟无枝酸菌新种
Reclassification of Amycolatopsis mediterranei DSM 46095 as Amycolatopsis rifamycinica sp. nov.
作者信息
Bala Shashi, Khanna Richie, Dadhwal M, Prabagaran S R, Shivaji S, Cullum John, Lal Rup
机构信息
Department of Zoology, University of Delhi, Delhi 110007, India.
Centre for Cellular and Molecular Biology, Hyderabad 500007, India.
出版信息
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1145-1149. doi: 10.1099/ijs.0.02901-0.
Previous experiments have suggested that the rifamycin-producing strain DSM 46095 might not belong to Amycolatopsis mediterranei. Analysis of its 16S rRNA gene sequence and construction of a phylogenetic tree showed most similarity to Amycolatopsis kentuckyensis NRRL B-24129T, Amycolatopsis lexingtonensis NRRL B-24129T and Amycolatopsis pretoriensis NRRL B-24133T, but the strain was probably not a member of any of these species. Results from DNA-DNA hybridization experiments and comparison of DNA profiling patterns using pulsed-field gel electrophoresis also supported the assignment of strain DSM 46095 to a novel species. Analyses of phospholipids, fatty acid methyl esters and physiological characteristics also showed that the differences between different isolates of A. mediterranei and A. mediterranei DSM 46095 were as large as those between Amycolatopsis species. Strain DSM 46095 represents a novel species of the genus Amycolatopsis for which the name Amycolatopsis rifamycinica sp. nov. is proposed, with the type strain NT 19T (=DSM 46095T=ATCC 27643T).
先前的实验表明，产利福霉素的菌株DSM 46095可能不属于地中海拟无枝酸菌。对其16S rRNA基因序列的分析以及系统发育树的构建显示，它与肯塔基拟无枝酸菌NRRL B - 24129T、列克星敦拟无枝酸菌NRRL B - 24129T和比勒陀利亚拟无枝酸菌NRRL B - 24133T最为相似，但该菌株可能不属于这些物种中的任何一种。DNA - DNA杂交实验结果以及使用脉冲场凝胶电泳对DNA谱型的比较也支持将菌株DSM 46095归为一个新物种。对磷脂、脂肪酸甲酯和生理特性的分析还表明，地中海拟无枝酸菌不同分离株与地中海拟无枝酸菌DSM 46095之间的差异与拟无枝酸菌属不同物种之间的差异一样大。菌株DSM 46095代表了拟无枝酸菌属的一个新物种，为此提出名称为利福霉素拟无枝酸菌（Amycolatopsis rifamycinica sp. nov.），模式菌株为NT 19T（=DSM 46095T=ATCC 27643T）。
Zotero 插件