Komarov E V, Ganin P G
Research Institute of Antibiotics and Medical Enzymes, Ministry of Industry, Science, and Technology of the Russian Federation, St. Petersburg, 198020 Russia.
Prikl Biokhim Mikrobiol. 2004 May-Jun;40(3):323-31.
Zeta-potential of n-alkane droplets, formed by fatty acids, were studied in model systems of culture liquid of yeasts (Candida maltosa) capable of utilizing n-alkanes. The value of zeta-potential was found to depend on the droplet size. The negative zeta-potential of submicron droplets was so high that it prevented the droplets from being coagulated with cells possessing a high negative zeta-potential. The dominant role of submicron n-alkane droplets in the kinetics of yeast growth could be accounted for by the existence of a mechanism regulating contact interactions of individual cells with the droplets followed by uptake of the substrate.
在能够利用正构烷烃的酵母(麦芽糖假丝酵母)培养液模型体系中,研究了由脂肪酸形成的正构烷烃液滴的ζ-电位。发现ζ-电位值取决于液滴大小。亚微米液滴的负ζ-电位非常高,以至于阻止了这些液滴与具有高负ζ-电位的细胞发生凝聚。亚微米正构烷烃液滴在酵母生长动力学中的主导作用,可以通过存在一种调节单个细胞与液滴的接触相互作用并随后摄取底物的机制来解释。