利用cDNA微阵列分析胃癌。

Analysis of gastric cancer with cDNA microarray.

作者信息

Haraguchi Naotsugu, Inoue Hiroshi, Mimori Koshi, Tanaka Fumiaki, Utsunomiya Tohru, Yoshikawa Kouji, Mori Masaki

机构信息

Department of Surgery and Molecular Oncology, Medical Institute of Bioregulation, Kyushu University, 4546 Tsurumihara, 874-0838 Beppu, Japan.

出版信息

Cancer Chemother Pharmacol. 2004 Sep;54 Suppl 1:S21-4. doi: 10.1007/s00280-004-0882-2.

DOI:10.1007/s00280-004-0882-2

PMID:15309510

Abstract

Recent advances in cDNA microarray techniques have enabled us to study the expression of many genes simultaneously. As gastric cancer remains one of the most common cancers in Japan, we studied gene expression profiles in gastric cancer by cDNA microarray analysis to determine if it would be clinically useful. We demonstrated two points. First, cDNA microarray might be useful as a prognostic indicator. However, there remain several important problems to be solved and these are discussed. Second, laser microdissection plus cDNA microarray might be useful in determining the specific genes that correlate to cancer metastasis or histological subtype. We review the pros and cons for cDNA microarray analysis in the context of gastric cancer.

摘要

cDNA微阵列技术的最新进展使我们能够同时研究许多基因的表达。由于胃癌仍是日本最常见的癌症之一，我们通过cDNA微阵列分析研究了胃癌中的基因表达谱，以确定其是否具有临床实用性。我们证实了两点。第一，cDNA微阵列可能作为一种预后指标。然而，仍有几个重要问题有待解决，本文将对此进行讨论。第二，激光显微切割加cDNA微阵列可能有助于确定与癌症转移或组织学亚型相关的特定基因。我们在胃癌的背景下综述了cDNA微阵列分析的利弊。