应用TaqMan探针通过物种特异性实时PCR对感染小麦中的禾谷镰刀菌进行定量分析。
Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan Probe.
作者信息
Reischer G H, Lemmens M, Farnleitner A, Adler A, Mach R L
机构信息
Institute for Chemical Engineering, Gene Technology Group, Vienna University of Technology, Getreidemarkt 9/166, A-1060 Vienna, Austria.
出版信息
J Microbiol Methods. 2004 Oct;59(1):141-6. doi: 10.1016/j.mimet.2004.06.003.
A new real-time PCR based method was developed for the species-specific detection, identification and quantification of Fusarium graminearum in planta. It utilizes a TaqMan hybridisation probe targeting the beta-tubulin gene and a plasmid standard. The assay is highly specific giving no product with DNA of closely related species. It is very sensitive, detecting down to five gene copies per reaction, and is able to produce reliable quantitative data over a range of six orders of magnitude.
开发了一种基于实时荧光定量PCR的新方法，用于在植物中对禾谷镰刀菌进行种特异性检测、鉴定和定量分析。该方法利用了一种靶向β-微管蛋白基因的TaqMan杂交探针和一个质粒标准品。该检测方法具有高度特异性，与近缘物种的DNA不产生扩增产物。它非常灵敏，每个反应能检测到低至5个基因拷贝，并且能够在六个数量级的范围内产生可靠的定量数据。
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