小鼠胚胎干细胞向特定神经谱系的分化。

Differentiation of mouse embryonic stem cells into a defined neuronal lineage.

作者信息

Bibel Miriam, Richter Jens, Schrenk Katrin, Tucker Kerry Lee, Staiger Volker, Korte Martin, Goetz Magdalena, Barde Yves-Alain

机构信息

Friedrich Miescher Institute, CH-4058 Basel, Switzerland.

出版信息

Nat Neurosci. 2004 Sep;7(9):1003-9. doi: 10.1038/nn1301.

DOI:10.1038/nn1301

PMID:15332090

Abstract

Although it has long been known that cultured embryonic stem cells can generate neurons, the lineage relationships with their immediate precursors remain unclear. We report here that selection of highly proliferative stem cells followed by treatment with retinoic acid generated essentially pure precursors that markers identified as Pax-6-positive radial glial cells. As they do in vivo, these cells went on to generate neurons with remarkably uniform biochemical and electrophysiological characteristics.

摘要

尽管长期以来人们都知道培养的胚胎干细胞能够生成神经元，但其与直接前体细胞的谱系关系仍不清楚。我们在此报告，选择高增殖性干细胞，然后用视黄酸处理，可产生基本上纯的前体细胞，标记物鉴定这些前体细胞为Pax-6阳性放射状胶质细胞。正如它们在体内的情况一样，这些细胞接着生成了具有显著一致的生化和电生理特征的神经元。