苛养腺病毒40型在HRT 18细胞中的生长：与C亚属腺病毒E1A和E1B缺失突变体的相互作用

Growth of fastidious adenovirus serotype 40 in HRT 18 cells: interactions with E1A and E1B deletion mutants of subgenus C adenoviruses.

作者信息

Gomes S A, Niel C, D'Halluin J C

机构信息

Departamento de Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.

出版信息

Arch Virol. 1992;124(1-2):45-56. doi: 10.1007/BF01314624.

DOI:10.1007/BF01314624

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7086997/

Abstract

Growth of fastidious adenovirus serotype 40 (Ad40) in several cell lines was investigated. Ad40 was able to readily propagate in human intestinal cell line, HRT 18. Coinfection assays were made in non-permissive and permissive cells between Ad40 and Ad5dl312 or dl1520, mutants deleted in E1A and E1B regions, respectively, to test the ability of Ad40 to complement these mutants and vice versa. Ad40 could enhance Ad5dl312 DNA synthesis in HRT18 and HeLa cells, although its own DNA disappeared in the presence of this mutant in HRT18 cells. In coinfection with dl1520, Ad40 DNA synthesis was inhibited by dl1520 in HRT18 cells and dl1520 DNA synthesis was inhibited by Ad40 in 293 cells. This might reflect the presence of unusual products encoded by Ad40 E1B region.

摘要

研究了苛养腺病毒血清型40（Ad40）在几种细胞系中的生长情况。Ad40能够在人肠道细胞系HRT 18中轻松繁殖。分别在Ad40与E1A和E1B区域缺失的突变体Ad5dl312或dl1520之间的非允许细胞和允许细胞中进行共感染试验，以测试Ad40互补这些突变体的能力以及反之亦然的能力。Ad40可以增强HRT18和HeLa细胞中Ad5dl312的DNA合成，尽管在HRT18细胞中，在这种突变体存在的情况下其自身的DNA消失了。在与dl1520共感染时，HRT18细胞中Ad40的DNA合成被dl1520抑制，而293细胞中dl1520的DNA合成被Ad40抑制。这可能反映了Ad40 E1B区域编码的异常产物的存在。

相似文献

1

Growth of fastidious adenovirus serotype 40 in HRT 18 cells: interactions with E1A and E1B deletion mutants of subgenus C adenoviruses.

Arch Virol. 1992;124(1-2):45-56. doi: 10.1007/BF01314624.

2

Complementation of enteric adenovirus type 40 for lytic growth in tissue culture by E1B 55K function of adenovirus types 5 and 12.

Virology. 1989 Aug;171(2):619-22. doi: 10.1016/0042-6822(89)90634-x.

3

Fastidious human adenovirus type 40 can propagate efficiently and produce plaques on a human cell line, A549, derived from lung carcinoma.

J Virol. 1991 May;65(5):2429-35. doi: 10.1128/JVI.65.5.2429-2435.1991.

4

Enteric adenovirus type 40: complementation of the E4 defect in Ad2 dl808.

Virology. 1991 Jul;183(1):433-6. doi: 10.1016/0042-6822(91)90161-4.

5

Functional relatedness between the E1a and E1b regions of group C and group D human adenoviruses.

Virus Res. 1987 Feb;7(1):33-48. doi: 10.1016/0168-1702(87)90056-6.

6

Structure and organization of the left-terminal DNA regions of fastidious adenovirus types 40 and 41.

Gene. 1987;58(1):109-26. doi: 10.1016/0378-1119(87)90034-5.

7

Enteric adenovirus type 40:E1B transcription map and identification of novel E1A-E1B cotranscripts in lytically infected cells.

Virology. 1991 Mar;181(1):139-49. doi: 10.1016/0042-6822(91)90478-t.

8

Characterization of adenovirus type 40 E1 region.

Virology. 1988 Jul;165(1):95-102. doi: 10.1016/0042-6822(88)90662-9.

9

The amino-terminal region of the adenovirus serotype 5 E1a protein performs two separate functions when expressed in primary baby rat kidney cells.

Mol Cell Biol. 1988 Sep;8(9):3882-90. doi: 10.1128/mcb.8.9.3882-3890.1988.

10

Expression of adenovirus E1B mutant phenotypes is dependent on the host cell and on synthesis of E1A proteins.

J Virol. 1987 Feb;61(2):426-35. doi: 10.1128/JVI.61.2.426-435.1987.

本文引用的文献

1

Mechanism of activation of early viral transcription by the adenovirus E1A gene product.

Cell. 1981 Oct;26(2 Pt 2):213-20. doi: 10.1016/0092-8674(81)90304-4.

2

In vitro growth of some fastidious adenoviruses from stool specimens.

J Clin Pathol. 1981 Feb;34(2):213-6. doi: 10.1136/jcp.34.2.213.

3

[A cell line particularly susceptible to bovine enteric Coronavirus replication: HRT 18 cells].

C R Seances Acad Sci D. 1980 Mar 3;290(9):623-6.

4

Characterization of an early temperature-sensitive and cytocidal double mutant of adenovirus type 2.

J Gen Virol. 1984 Aug;65 ( Pt 8):1305-17. doi: 10.1099/0022-1317-65-8-1305.

5

Mutations in the gene encoding the adenovirus early region 1B 19,000-molecular-weight tumor antigen cause the degradation of chromosomal DNA.

J Virol. 1984 Nov;52(2):410-9. doi: 10.1128/JVI.52.2.410-419.1984.

6

Candidate adenoviruses 40 and 41: fastidious adenoviruses from human infant stool.

J Med Virol. 1983;11(3):215-31. doi: 10.1002/jmv.1890110305.

7

Early replicative block prevents the efficient growth of fastidious diarrhea- associated adenoviruses in cell culture.

J Med Virol. 1982;9(2):93-100. doi: 10.1002/jmv.1890090203.

8

Restriction endonucleases in identification of a genome type of adenovirus 19 associated with keratoconjunctivitis.

Infect Immun. 1980 Feb;27(2):292-6. doi: 10.1128/iai.27.2.292-296.1980.

9

Propagation and in vitro studies of previously non-cultivable enteral adenoviruses in 293 cells.

Lancet. 1981 Oct 17;2(8251):832-4. doi: 10.1016/s0140-6736(81)91104-1.

10

Genetic expression of human adenoviruses in simian cells. Evidence for interserotypic inhibition of viral DNA synthesis.

Virus Res. 1984;1(5):365-80. doi: 10.1016/0168-1702(84)90024-8.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。