Ogarkova O A, Tomilov A A, Tomilova N B, Tarasov V A
Ontogenez. 2004 May-Jun;35(3):220-8.
A group of 13 recessive lethal mutants was selected on the basis of the collection of Arabidopsis thaliana transgenic plants with insertions of T-DNA vector plasmid pLD3 or pPCVRN4, which was produced by agrobacterial transformation of germinating seeds. The use of media containing exogenous hormones made it possible to compensate the lethal effect, identify phenotypes, and characterize six lines of recessive lethal germlings using genetic and molecular-genetic methods.
基于通过农杆菌介导的萌发种子转化产生的插入T-DNA载体质粒pLD3或pPCVRN4的拟南芥转基因植物群体,挑选出了一组13个隐性致死突变体。使用含有外源激素的培养基能够补偿致死效应、鉴定表型,并利用遗传学和分子遗传学方法对6个隐性致死幼苗品系进行表征。