从地钱（Bazzania trilobata (L.) S.F. Gray）中进行生物活性导向的抗真菌化合物分离。

Bioactivity guided isolation of antifungal compounds from the liverwort Bazzania trilobata (L.) S.F. Gray.

作者信息

Scher Jochen M, Speakman John-Bryan, Zapp Josef, Becker Hans

机构信息

FR 8.7, Pharmakognosie und Analytische Phytochemie, Universität des Saarlandes, Postfach 151150, D-66041 Saarbrücken, Germany.

出版信息

Phytochemistry. 2004 Sep;65(18):2583-8. doi: 10.1016/j.phytochem.2004.05.013.

DOI:10.1016/j.phytochem.2004.05.013

PMID:15451321

Abstract

A dichloromethane and a methanol extract of the liverwort Bazzania trilobata showed antifungal activity against the phytopathogenic fungi Botrytis cinerea, Cladosporium cucumerinum, Phythophthora infestans, Pyricularia oryzae and Septoria tritici. Bioautography on thin-layer chromatograms was used to isolate six antifungal sesquiterpenes: 5- and 7-hydroxycalamenene, drimenol, drimenal, viridiflorol, gymnomitrol and three bisbibenzyls: 6 ',8'-dichloroisoplagiochin C, isoplagiochin D and 6'-chloroisoplagiochin D. Furthermore we report the isolation of gymnomitr-8(12)-en-4-one and the new coumarin 7,8-dihydroxycoumarin-7-O-beta-D-glucuronide. Their structures have been elucidated based on extensive NMR spectral evidence.

摘要

地钱（Bazzania trilobata）的二氯甲烷提取物和甲醇提取物对植物病原真菌灰葡萄孢（Botrytis cinerea）、黄瓜枝孢（Cladosporium cucumerinum）、致病疫霉（Phythophthora infestans）、稻瘟病菌（Pyricularia oryzae）和小麦壳针孢（Septoria tritici）显示出抗真菌活性。利用薄层色谱进行生物自显影，分离出六种抗真菌倍半萜：5-羟基和7-羟基菖蒲烯、德瑞木醇、德瑞木醛、绿花烯、裸蕨醇以及三种联苄：6',8'-二氯异普拉吉奥钦C、异普拉吉奥钦D和6'-氯异普拉吉奥钦D。此外，我们还报告了裸蕨-8(12)-烯-4-酮和新香豆素7,8-二羟基香豆素-7-O-β-D-葡萄糖醛酸苷的分离。基于大量核磁共振光谱证据阐明了它们的结构。