位于3号、8号和9号染色体上的数量性状基因座调控小鼠针对沙门氏菌鞭毛抗原的抗体产生。

Quantitative trait loci in Chromosomes 3, 8, and 9 regulate antibody production against Salmonella flagellar antigens in the mouse.

作者信息

de Souza Carla M, Morel Laurence, Cabrera Wafa H K, Starobinas Nancy, Ribeiro Orlando G, Siqueira Maria, Ibañez Olga M, De Franco Marcelo

机构信息

Laboratório de Imunogenética, Instituto Butantan, Av. Vital Brazil, 1500, São Paulo, 05503900, SP.

出版信息

Mamm Genome. 2004 Aug;15(8):630-6. doi: 10.1007/s00335-004-2378-0.

DOI:10.1007/s00335-004-2378-0

PMID:15457342

Abstract

Two mouse lines were produced by bidirectional selection according to the high (HIII) or low (LIII) antibody responsiveness against Salmonella flagellar antigens (Selection III). In the present work we conducted a genomewide scan to map the quantitative trait loci (QTL) involved in the antibody response regulation in these selected mice. HIII and LIII genomes were screened with microsatellite markers and those found polymorphic between the lines (146) were used for linkage analysis in F2 (HIII x LIII) intercross. Simple interval mapping analysis was performed using Mapmanager QTX software. Three highly significant QTL linked to antibody production against Salmonella flagellar antigens have been demonstrated in Chromosomes 3, 8, and 9. HIII and LIII lines differ in the resistance to several diseases, therefore, the relevance of these QTL with the genetic factors involved in infections, autoimmunity, and neoplastic disease progression is discussed.

摘要

根据对沙门氏菌鞭毛抗原的高（HIII）或低（LIII）抗体反应性，通过双向选择培育了两个小鼠品系（选择III）。在本研究中，我们进行了全基因组扫描，以绘制这些选择小鼠中参与抗体反应调节的数量性状基因座（QTL）。用微卫星标记筛选HIII和LIII基因组，并将那些在品系间发现多态性的标记（146个）用于F2（HIII×LIII）杂交的连锁分析。使用Mapmanager QTX软件进行简单区间定位分析。已在3号、8号和9号染色体上证实了三个与针对沙门氏菌鞭毛抗原的抗体产生相关的高度显著QTL。HIII和LIII品系在对几种疾病的抗性方面存在差异，因此，讨论了这些QTL与涉及感染、自身免疫和肿瘤疾病进展的遗传因素的相关性。