一种使用液相色谱法快速测定植物药口服液中补骨脂素的方法。

A method for fast determination of psoralens in oral solutions of phytomedicines using liquid chromatography.

作者信息

Pires Adriana Elias, Honda Neli Kiko, Cardoso Cláudia Andréa Lima

机构信息

Departamento de Química, Universidade Federal de Mato Grosso do Sul, Caixa Postal 649, 79070-900 Campo Grande/MS, Brazil.

出版信息

J Pharm Biomed Anal. 2004 Oct 29;36(2):415-20. doi: 10.1016/j.jpba.2004.06.019.

DOI:10.1016/j.jpba.2004.06.019

PMID:15496338

Abstract

A method for sample preparation and analysis by high performance liquid chromatography with UV detection (HPLC-UV) has been developed for routine analysis of psoralen and bergapten, photosensitizing compounds, in oral solutions of phytomedicines employed in Brazil for some illnesses. The linearity, accuracy, the inter- and intra-day precision of the procedure were evaluated. Calibration curves for psoralen and bergapten were linear in the range of 1.0-600.0 microg ml(-1) and 1.0-400.0 microg ml(-1) respectively. The recoveries of the psoralens in the oral solutions analysed were 94.43-99.97%. The percentage coefficient of variation (CV) of the quantitative analysis of the psoralens in the products analysis was within 5%. In inter-equipment study was employed gas chromatography-flame ionization (CG-FID) detection.

摘要

已开发出一种通过高效液相色谱-紫外检测（HPLC-UV）进行样品制备和分析的方法，用于对巴西用于某些疾病的植物药口服液中的补骨脂素和佛手柑内酯这两种光敏化合物进行常规分析。评估了该方法的线性、准确性以及日内和日间精密度。补骨脂素和佛手柑内酯的校准曲线分别在1.0 - 600.0 μg ml⁻¹和1.0 - 400.0 μg ml⁻¹范围内呈线性。所分析的口服液中补骨脂素的回收率为94.43% - 99.97%。产品分析中补骨脂素定量分析的变异系数（CV）百分比在5%以内。在设备间研究中采用了气相色谱-火焰离子化（CG-FID）检测。