老年人类牙髓中连接蛋白43表达的降低
Reduction of connexin 43 expression in aged human dental pulp.
作者信息
Muramatsu T, Hamano H, Ogami K, Ohta K, Inoue T, Shimono M
机构信息
Oral Health Science Center, Department of Pathology, Tokyo Dental College, Chiba, Japan.
出版信息
Int Endod J. 2004 Dec;37(12):814-8. doi: 10.1111/j.1365-2591.2004.00880.x.
AIM
To investigate the expression of connexin 43 (CX43) mRNA in young and old human dental pulp tissues to determine the characteristics of CX43 expression.
METHODOLOGY
Samples were obtained from human dental pulp of healthy young (17-23 years) and aged (>50 years) subjects. CX43 expression was determined by RT-PCR and by quantitative real-time RT-PCR (QRT-PCR). The threshold cycle (Ct) value, which reflects the amount of PCR, was calculated and the difference between value in the young pulp and that in the aged pulp was statistically analysed.
RESULTS
RT-PCR analysis of human dental pulp tissue detected CX43 mRNA in all the samples. CX43 was abundantly expressed in young adult dental pulp, but expression of CX43 mRNA was dramatically decreased in aged human dental pulp. QRT-PCR analysis also showed the reduced expression of CX43 in aged pulp, and expression of CX43 in young pulp was significantly higher (about 10-fold, P < 0.01, Mann-Whitney U-test).
CONCLUSION
Reduction of CX43 expression may be associated with the loss of viability in human dental pulp, and is considered as one characteristic of aged pulp.
目的
研究连接蛋白43（CX43）mRNA在年轻和老年人类牙髓组织中的表达情况，以确定CX43表达的特征。
方法
从健康年轻（17 - 23岁）和老年（>50岁）受试者的人类牙髓中获取样本。通过逆转录聚合酶链反应（RT-PCR）和定量实时逆转录聚合酶链反应（QRT-PCR）测定CX43的表达。计算反映PCR量的阈值循环（Ct）值，并对年轻牙髓和老年牙髓中的值差异进行统计学分析。
结果
对人类牙髓组织的RT-PCR分析在所有样本中均检测到CX43 mRNA。CX43在年轻成人牙髓中大量表达，但在老年人类牙髓中CX43 mRNA的表达显著降低。QRT-PCR分析也显示老年牙髓中CX43表达降低，且年轻牙髓中CX43的表达明显更高（约10倍，P < 0.01，曼-惠特尼U检验）。
结论
CX43表达的降低可能与人类牙髓活力丧失有关，并被认为是老年牙髓的一个特征。
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