前胸腺素α与癌蛋白SET相关联，并参与染色质解聚。

Prothymosin alpha associates with the oncoprotein SET and is involved in chromatin decondensation.

作者信息

Karetsou Zoe, Martic Goran, Tavoulari Sotiria, Christoforidis Savvas, Wilm Matthias, Gruss Claudia, Papamarcaki Thomais

机构信息

Laboratory of Biological Chemistry, Medical School, University of Ioannina, 451 10 Ioannina, Greece.

出版信息

FEBS Lett. 2004 Nov 19;577(3):496-500. doi: 10.1016/j.febslet.2004.09.091.

DOI:10.1016/j.febslet.2004.09.091

PMID:15556635

Abstract

Prothymosin alpha (ProTalpha) is a histone H1-binding protein that interacts with the transcription coactivator CREB-binding protein and potentiates transcription. Based on coimmunoprecipitation and mammalian two-hybrid assays, we show here that ProTalpha forms a complex with the oncoprotein SET. ProTalpha efficiently decondenses human sperm chromatin, while overexpression of GFP-ProTalpha in mammalian cells results in global chromatin decondensation. These results indicate that decondensation of compacted chromatin fibers is an important step in the mechanism of ProTalpha function.

摘要

前胸腺素α（ProTα）是一种与组蛋白H1结合的蛋白质，它与转录共激活因子CREB结合蛋白相互作用并增强转录。基于免疫共沉淀和哺乳动物双杂交分析，我们在此表明ProTα与癌蛋白SET形成复合物。ProTα能有效地使人类精子染色质解聚，而在哺乳动物细胞中过表达绿色荧光蛋白标记的ProTα会导致整体染色质解聚。这些结果表明，紧密染色质纤维的解聚是ProTα发挥功能机制中的一个重要步骤。