[Studies on sex identification and variation of endogenous hormones in female and male plants of Gynostemma pentaphyllum].

OBJECTIVE

To distinguish female and male plants of Gynostemma pentaphyllum quickly and accurately in the stage of seedling and to understand the relation between sex differentiation and endogenous plant hormone.

METHOD

Stained methods of BTB and enzyme linked immuno-sorbent Assay were used.

RESULT

10 Hours after being dyed, the liquid extract of female plants turned into yellow, while extract of male ones turned green. There were obvious differences on iPAs and GA(1 + 3) contents but no differences on IAA and ABA contents between male and female plants during the stages of seedling and vegetative growth.

CONCLUSION

BTB can be used for distinguishing female and male plants and there were a certain relation between sex and endogenous plant hormones.