检测在医院或社区死亡的疑似免疫功能正常婴儿中的卡氏肺孢子虫人型变种和病毒。
Detection of Pneumocystis carinii f. sp. hominis and viruses in presumably immunocompetent infants who died in the hospital or in the community.
作者信息
Vargas Sergio L, Ponce Carolina A, Luchsinger Vivian, Silva Cecilia, Gallo Myriam, López René, Belletti José, Velozo Luis, Avila Ricardo, Palomino Maria A, Benveniste Samuel, Avendaño Luis F
机构信息
Programas de Microbiologia, Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
出版信息
J Infect Dis. 2005 Jan 1;191(1):122-6. doi: 10.1086/426451. Epub 2004 Dec 6.
Fresh-frozen lung and tracheal-aspirate specimens obtained from 112 infants who died in Santiago, Chile, during 1998-2000 were analyzed for the presence of Pneumocystis DNA, by use of nested DNA amplification of the large subunit mitochondrial rRNA, and for the presence of viruses, by use of culture and immunofluorescence. Pneumocystis DNA was detected in specimens from 45 (51.7%) of 87 infants who died in the community and from 5 (20%) of 25 infants who died in the hospital (P=.006). Primary infection with Pneumocystis was highly frequent among infants who die unexpectedly in the community. Infection with viruses was more common in infants who died in the hospital.
对1998年至2000年期间在智利圣地亚哥死亡的112例婴儿的新鲜冷冻肺和气管吸出物标本进行分析，采用线粒体大核糖体RNA的巢式DNA扩增检测肺孢子菌DNA的存在，并采用培养和免疫荧光法检测病毒的存在。在社区死亡的87例婴儿中，有45例（51.7%）的标本检测到肺孢子菌DNA；在医院死亡的25例婴儿中，有5例（20%）检测到（P=0.006）。在社区意外死亡的婴儿中，肺孢子菌原发性感染非常常见。病毒感染在医院死亡的婴儿中更为常见。
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