使用石英晶体微天平（QCM）和电化学指示剂方法检测适体与蛋白质的相互作用。

Detection of aptamer-protein interactions using QCM and electrochemical indicator methods.

作者信息

Hianik Tibor, Ostatná Veronika, Zajacová Zuzana, Stoikova Ekaterina, Evtugyn Gennady

机构信息

Faculty of Mathematics, Physics and Computer Sciences, Comenius University, 842 48 Bratislava, Slovakia.

出版信息

Bioorg Med Chem Lett. 2005 Jan 17;15(2):291-5. doi: 10.1016/j.bmcl.2004.10.083.

DOI:10.1016/j.bmcl.2004.10.083

PMID:15603942

Abstract

We report novel method of detection thrombin-aptamer interaction based on measurement the charge consumption from the electrode covered by DNA aptamers to an electrochemical indicator methylene blue (MB), that is bounded to a thrombin. The binding of thrombin to an aptamers has been detected also by QCM method in flow measuring cell. We showed that using MB it is possible to detect thrombin with high sensitivity and selectivity.

摘要

我们报道了一种基于测量覆盖有DNA适配体的电极向与凝血酶结合的电化学指示剂亚甲基蓝（MB）的电荷消耗来检测凝血酶-适配体相互作用的新方法。凝血酶与适配体的结合也已通过流动测量池中的QCM方法检测到。我们表明，使用MB可以高灵敏度和高选择性地检测凝血酶。