伯氏疏螺旋体的质粒：一种病原体的必需遗传元件。

The plasmids of Borrelia burgdorferi: essential genetic elements of a pathogen.

作者信息

Stewart Philip E, Byram Rebecca, Grimm Dorothee, Tilly Kit, Rosa Patricia A

机构信息

Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th St., Hamilton, MT 59840, USA.

出版信息

Plasmid. 2005 Jan;53(1):1-13. doi: 10.1016/j.plasmid.2004.10.006. Epub 2004 Dec 16.

DOI:10.1016/j.plasmid.2004.10.006

PMID:15631949

Abstract

The spirochete Borrelia burgdorferi, the causative agent of Lyme disease, has an unusual genome comprised of a linear chromosome and the largest plasmid complement of any characterized bacterium. Certain plasmid-encoded elements are required for virulence and viability, both in vitro and in vivo. The genetic tools to manipulate B. burgdorferi are sufficiently developed for precise molecular genetic investigations. B. burgdorferi now represents a prime system with which to address basic questions of plasmid biology and plasmid contributions to bacterial virulence and disease pathogenesis.

摘要

莱姆病的病原体——螺旋体伯氏疏螺旋体，具有一个不同寻常的基因组，它由一条线性染色体和任何已鉴定细菌中最大的质粒互补体组成。某些质粒编码元件在体外和体内对于毒力和生存能力都是必需的。用于操纵伯氏疏螺旋体的遗传工具已经得到充分发展，可用于精确的分子遗传学研究。伯氏疏螺旋体现在是一个主要系统，可用于解决质粒生物学的基本问题以及质粒对细菌毒力和疾病发病机制的作用。