Wu Fang, Qu Bin, Wang Xue-Feng, Yang Chen-Min, Wang Hong-Li
Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Zhonghua Nei Ke Za Zhi. 2004 Nov;43(11):837-40.
To study the changes of hemostatic molecular markers in patients with gastric or intestinal cancer for elucidating their clinical significance.
The plasma levels of tissue factor (TF), thrombin antithrombin complex (TAT), tissue plasminogen activator (t-PA), urokinase plasminogen activator (u-PA), urokinase plasminogen activator receptor (u-PAR) and plasmin antiplasmin complex (PAP) were measured by ELISA. Gene transcription of TF, t-PA, u-PA mRNA were detected by real-time RT-PCR.
The plasma levels of TF, TAT, u-PA, u-PAR and PAP were elevated in gastric or intestinal cancer patients (P < 0.05), while u-PA, u-PAR remarkably increased in patients with local infiltration, lymph node involvement or distal metastasis (P < 0.01). Plasma level of TF, TAT, PAP were remained higher than control even after surgery. TF, u-PA mRNA were higher (P < 0.01) and t-PA was lower (P > 0.05) in gastric or intestinal cancer compared to normal tissue.
Their existed over expression of TF and u-PA, increasing formation of thrombin and plasminogen in gastric or intestinal cancer patients. Hypercoagulability and hyperfibrinolysis were important factors related with metastasis potential of gastric or intestinal cancer. t-PA may be a character of well differentiated tissue. Quantitative detection of TF and u-PA gene expression by the method of real-time RT-PCR is feasible for them as observation markers in gastric or intestinal cancer patients.
研究胃肠道癌症患者止血分子标志物的变化，以阐明其临床意义。
采用酶联免疫吸附测定法（ELISA）检测血浆组织因子（TF）、凝血酶抗凝血酶复合物（TAT）、组织型纤溶酶原激活剂（t-PA）、尿激酶型纤溶酶原激活剂（u-PA）、尿激酶型纤溶酶原激活剂受体（u-PAR）和纤溶酶抗纤溶酶复合物（PAP）水平。通过实时逆转录聚合酶链反应（real-time RT-PCR）检测TF、t-PA、u-PA mRNA的基因转录情况。
胃肠道癌症患者血浆中TF、TAT、u-PA、u-PAR和PAP水平升高（P < 0.05），而局部浸润、淋巴结受累或远处转移患者的u-PA、u-PAR显著升高（P < 0.01）。即使手术后，TF、TAT、PAP的血浆水平仍高于对照组。与正常组织相比，胃肠道癌症中TF、u-PA mRNA较高（P < 0.01），t-PA较低（P > 0.05）。
胃肠道癌症患者存在TF和u-PA的过表达，凝血酶和纤溶酶原形成增加。高凝性和高纤溶状态是与胃肠道癌症转移潜能相关的重要因素。t-PA可能是高分化组织的一个特征。采用实时RT-PCR方法定量检测TF和u-PA基因表达，作为胃肠道癌症患者的观察指标是可行的。
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